000			04186nam a22003735i 4500
001			234706
003			ES-VaUE
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007			cr nn 008mamaa
008			151117s2016 xxu| s |||| 0|eng d
020			_a9781493931156
024	7		_a10.1007/978-1-4939-3115-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPlant Signal Transduction _bMethods and Protocols _cedited by Jose R. Botella, Miguel A. Botella.
250			_a2nd edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XV, 241 páginas) _b41 ilustraciones, 25 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1363
505	0		_aSuppressor Screens in Arabidopsis -- Quantitative Reverse Transcription-qPCR-Based Gene Expression Analysis in Plants -- DNA-Binding Factor Target Identification by Chromatin Immunoprecipitation (ChIP) in Plants -- Identification of Genes Responsible for Natural Variation in Volatile Content Using Next Generation Sequencing Technology -- Micro-Tom Tomato as an Alternative Plant Model System: Mutant Collection and Efficient Transformation -- Culture of the Tomato Micro-Tom Cultivar in Greenhouse -- Virus-Induced Gene Silencing as a Tool to Study Tomato Fruit Biochemistry -- A Simplified and Rapid Method for the Isolation and Transfection of Arabidopsis Leaf Mesophyll Protoplasts for Large-Scale Applications -- Preparation of Epidermal Peels and Guard Cell Protoplasts for Cellular, Electrophysiological, and -Omics Assays of Guard Cell Function -- Application of Variable Angle Total Internal Reflection Fluorescence Microscopy to Investigate Protein Dynamics in Intact Plant Cells -- Immunoprecipitation of Plasma Membrane Receptor-Like Kinases for Identification of Phosphorylation Sites and Associated Proteins -- Yeast Three-Hybrid System for the Detection of Protein-Protein Interactions -- Cautions in Measuring In Vivo Interactions Using FRET and BiFC in Nicotiana benthamiana -- Analysis of Protein-Lipid Interactions Using Purified C2 Domains -- Assessing Kinase Activity in Plants with In-Gel Kinase Assays -- Analyses of Plant UDP-Dependent Glycosyltransferases to Identify Their Volatile Substrates Using Recombinant Proteins -- Competitive Index: Mixed Infection-Based Virulence Assays for Genetic Analysis in Pseudomonas syringae-Plant Interactions -- Simplified Assays for Evaluation of Resistance to Alternaria brassicicola and Turnip Mosaic Virus -- Simultaneous Determination of Plant Hormones by GC-TOF-MS.
520			_aThis fully updated volume reflects the spectacular advances in our knowledge of signal transduction pathways with a selection of 'classic' as well as newly developed approaches. These detailed approaches expand into the fields of molecular biology, biochemistry, physiology, cell biology, genetics, and genomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Practical and up-to-date, Plant Signal Transduction: Methods and Protocols, Second Edition serves as an ideal guide for researchers exploring the vast array of signals produced by plants to ensure their survival.
700	1		_aBotella, Jose R _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aBotella, Miguel A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493931149
776	0	8	_iPrinted edition: _z9781493931163
776	0	8	_iPrinted edition: _z9781493949595
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3115-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c234706 _d234706