000			03812nam a22003735i 4500
001			234723
003			ES-VaUE
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007			cr nn 008mamaa
008			120209s2012 xxu| s |||| 0|eng d
020			_a9781617795671
024	7		_a10.1007/978-1-61779-567-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aSingle-Cell Analysis _bMethods and Protocols _cedited by Sara Lindström, Helene Andersson-Svahn.
250			_a1st edition 2012
264		1	_aTotowa, NJ _bHumana Press _c2012
300			_a1 recurso en línea (X, 238 páginas) _b63 ilustraciones, 16 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v853
505	0		_aIntroduction: Why Analyze Single Cells? -- Flow Cytometry and Microscopy as Means of Studying Single Cells: A Short Introductional Overview -- Identification of Enzyme-Converted Peptide Products from Single Cells Using Capillary Electrophoresis and Liquid Chromatography-Mass Spectrometry -- Laser Capture Microdissection for Gene Expression Analysis of Specific Cell Populations in Single Blastocysts -- Single-Cell Culture in Microwells -- Iso-Dielectric Separation and Analysis of Cells -- Single Cell Electroporation Using Microfluidic Devices -- Perfusion Culture of Mammalian Cells in a Microfluidic Channel with a Build-In Pillar Array -- Padlock Probes and Rolling Circle Amplification for Detection of Repeats and Single-Copy Genes in the Single-Cell Comet Assay -- Droplet Microfluidics for Single-Cell Analysis -- Screening of Antigen-Specific Antibody Secreting Cells -- Analysis of Single Eukaryotic Cells Using Raman Tweezers -- Single-Cell Microinjection Technologies -- Ultrasonic Manipulation of Single Cells -- Expanding the Horizons for Single-Cell Applications on Lab-On-a-Chip Devices -- Analytical Technologies for Integrated Single-Cell Analysis of Human Immune Responses.
520			_aToday, cells are commonly analyzed en masse, with thousands of cells per sample, yielding results on the average response of the cells. However, cellular heterogeneity implies that in order to learn more about cellular behaviour, it is important to study how individual cells respond, one by one. In Single-Cell Analysis: Methods and Protocols, experts in the field provide an update on the field of single-cell analysis wherein the latest findings and applications are described in detail.  The methods described in this book include a few examples of conventional methods and several examples of miniaturized methods.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and accessible, Single-Cell Analysis: Methods and Protocols encourages readers to explore new ways of studying cells that may help lead to exciting new discoveries.
700	1		_aLindström, Sara _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aAndersson-Svahn, Helene _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617795688
776	0	8	_iPrinted edition: _z9781617795664
776	0	8	_iPrinted edition: _z9781493959211
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-567-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2012
999			_c234723 _d234723