000			03633nam a22003615i 4500
001			234737
003			ES-VaUE
005			20221220020622.0
007			cr nn 008mamaa
008			160614s2016 xxu| s |||| 0|eng d
020			_a9781493936526
024	7		_a10.1007/978-1-4939-3652-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMolecular Genetics of Asthma _cedited by María Isidoro García.
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XV, 275 páginas) _b43 ilustraciones, 38 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1434
505	0		_aApplications of Molecular Genetics to the Study of Asthma -- Assessment and Validation of New Genetic Variants: A systematic In Silico Approach -- Introduction to the Gene Expression Analysis -- Real Time PCR for Gene Expression Quantification in Asthma -- Validation of Reference Genes in mRNA Expression Analysis Applied to the Study of Asthma -- Review of Methods to Study Gene Expression Regulation Applied to Asthma -- Interactions of DNA and Proteins: Electrophoretic Mobility Shift Assay in Asthma -- SDS-Polyacrylamide Electrophoresis and Western Blotting Applied to the Study of Asthma -- Chromatin Immunoprecipitation: Application to the Study of Asthma -- Gene Silencing Delivery Methods: Lipid-Mediated and Electroporation Transfection Protocols -- Protocols for Exosome Isolation and RNA Profiling -- Cell Culture Techniques: Corticosteroid Treatment in A549 Human Lung Epithelial Cell -- Protocol for Lipid-Mediated Transient Transfection in A549 Epithelial Lung Cell Line -- Promoter Assay Using Luciferase Reporter Gene in the A549 Cell Line -- Review of Mouse Models Applied to the Study of Asthma -- General and Specific Mouse Models for Asthma Research -- Mouse Models applied to the Research of Pharmacological Treatments in Asthma -- Review on Pharmacogenetics and Pharmacogenomics Applied to the Study of Asthma.
520			_aPresenting topics from the basic application of molecular genetics to more complex gene expression analysis using different models of study, this detailed volume explores asthma through the lens of genetics, considered to play an essential role in the etiopathogenesis of the disease. Since asthma is a complex disease, this book is designed to provide a review of the most useful techniques with examples of their applications in specific laboratory protocols. Written in the highly successfulMethods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Molecular Genetics of Asthma serves as an ideal guide to researchers investigating this vital field of study.
700	1		_aIsidoro García, María _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493936502
776	0	8	_iPrinted edition: _z9781493936519
776	0	8	_iPrinted edition: _z9781493981076
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3652-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c234737 _d234737