000			04671nam a22003495i 4500
001			234745
003			ES-VaUE
005			20221220020622.0
007			cr nn 008mamaa
008			100301s2005 xxu| s |||| 0|eng d
020			_a9781592598236
024	7		_a10.1385/1592598234 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aOligonucleotide Synthesis _bMethods and Applications _cedited by Piet Herdewijn.
250			_a1st edition 2005
264		1	_aTotowa, NJ _bHumana Press _c2005
300			_a1 recurso en línea (XVI, 436 páginas) _b142 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v288
505	0		_aSynthesis of DNA Using a New Two-Step Cycle -- RNA Synthesis Using 2?-O-(Tert-Butyldimethylsilyl) Protection -- RNA Oligonucleotide Synthesis Via 5?-Silyl-2?-Orthoester Chemistry -- Dimethylthiarum Disulfide -- Assay for Evaluating Ribonuclease H-Mediated Degradation of RNA-Antisense Oligonucleotide Duplexes -- Di- and Oligonucleotide Synthesis Using H-Phosphonate Chemistry -- Solid-Phase Synthesis of Circular Oligonucleotides -- Locked Nucleic Acid Synthesis -- Synthesis of DNA Mimics Representing HypNA-pPNA Hetero-Oligomers -- Base-Modified Oligonucleotides With Increased Duplex Stability -- of Hypermodified Nucleotides in RNA -- Chemical Methods for Peptide-Oligonucleotide Conjugate Synthesis -- Biotin-Labeled Oligonucleotides With Extraordinarily Long Tethering Arms -- Universal Labeling Chemistry for Nucleic Acid Detection on DNA Chips -- Postsynthetic Functionalization of Triple Helix-Forming Oligonucleotides -- Fluorescence-Based On-Line Detection as an Analytical Tool in RNA Electrophoresis -- Design and Optimization of Molecular Beacon Real-Time Polymerase Chain Reaction Assays -- High-Throughput Production of Optimized Primers (Fimers) for Whole-Genome Direct Sequencing -- Nonenzymatic Template-Directed RNA Synthesis -- DNase I Footprinting of Small Molecule Binding Sites on DNA -- Gene Targeting Using Peptide Nucleic Acid -- Nucleic Acid Library Construction Using Synthetic DNA Constructs -- In Vitro Selection From Combinatorial Nucleic Acid Libraries -- In Vitro Selection Procedures for Identifying DNA and RNA Aptamers Targeted to Nucleic Acids and Proteins -- Short Double-Stranded Ribonucleic Acid as Inhibitor of Gene Expression by the Interference Mechanism.
520			_aNucleic acids chemistry has progressed rapidly in the last 10 years with the introduction of many new techniques and the development of many novel therapeutic entities. In Oligonucleotide Synthesis: Methods and Applications, laboratory experts describe in step-by-step detail the powerful new techniques they have developed for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are the synthesis of phosphorothioates via new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, triple helix formation, and a novel two-step process that yields a high purity DNA that is less costly. Novel applications include the use of molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules to DNA, nucleic acid libraries, optimization of RNA synthesis for siRNA applications, and the use of small interference RNA (siRNA) as an inhibitor of gene expression. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Oligonucleotide Synthesis: Methods and Applications offers today's investigators not only insight into important new biotechnologies, but also a full range of the detailed protocols needed to work successfully in DNA synthesis and its applications today.
700	1		_aHerdewijn, Piet _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617374418
776	0	8	_iPrinted edition: _z9781588292339
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592598234 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2005
999			_c234745 _d234745