000			04091nam a22003735i 4500
001			234747
003			ES-VaUE
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007			cr nn 008mamaa
008			140916s2014 xxu| s |||| 0|eng d
020			_a9781493910595
024	7		_a10.1007/978-1-4939-1059-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aBrain Energy Metabolism _cedited by Johannes Hirrlinger, Helle S. Waagepetersen.
250			_a1st edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (XIV, 368 páginas) _b97 ilustraciones, 52 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aNeuromethods _x1940-6045 _v90
505	0		_aDetermination of CO2 Production in Subcellular Preparations like Synaptosomes and Isolated Mitochondria Using 14C-Labeled Substrates and Radioactive CO2 Measurements -- Transport of Lactate: Characterization of the Transporters Involved in Transport at the Plasma Membrane by Heterologous Protein Expression in Xenopus Oocytes -- Primary Cultures of Astrocytes and Neurons as Model Systems to Study the Metabolism and Metabolite Export from Brain Cells -- Metabolic Mapping of Astrocytes and Neurons in Culture Using Stable Isotopes and Gas Chromatography-Mass Spectrometry (GC-MS) -- Metabolic Flux Analysis Tools to Investigate Brain Metabolism In Vitro -- Fluorescent Nanosensor Based Flux Analysis: Overview and the Example of Glucose -- Mitochondrial Bioenergetics Assessed by Functional Fluorescence Dyes -- RNA Interference as a Tool to Selectively Down-Modulate Protein Function -- Localizing and Quantifying Metabolites In Situ with Luminometry: Induced Metabolic Bioluminescence Imaging (imBI) -- A Chip Off the Old Block: The Brain Slice as a Model for Metabolic Studies of Brain Compartmentation and Neuropharmacology -- Integrated Measurements of Electrical Activity, Oxygen Tension, Blood Flow, and Ca2+-Signaling in Rodents In Vivo -- Measuring Cerebral Hemodynamics and Energy Metabolism by Near-Infrared Spectroscopy -- Compartmental Analysis of Metabolism by 13C Magnetic Resonance Spectroscopy -- Positron Emission Tomography of Brain Glucose Metabolism with [18F]Fluorodeoxyglucose in Humans.
520			_aBrain Energy Metabolism addresses its challenging subject by presenting diverse technologies allowing for the investigation of brain energy metabolism on different levels of complexity. Model systems are discussed, starting from the reductionist approach like primary cell cultures which allow assessing of the properties and functions of a single brain cell type with many different types of analysis, however, at the expense of neglecting the interaction between cell types in the brain. On the other end, analysis in animals and humans in vivo is discussed, maintaining the full complexity of the tissue and the organism but making high demands on the methods of analysis. Written for the popular Neuromethods series, chapters include the kind of detailed description and key implementation advice that aims to support reproducible results in the lab.   Meticulous and authoritative, Brain Energy Metabolism provides an ideal guide for researchers interested in brain energy metabolism with the hope of stimulating more research in this exciting and very important field.
700	1		_aHirrlinger, Johannes _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aWaagepetersen, Helle S _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493910601
776	0	8	_iPrinted edition: _z9781493910588
776	0	8	_iPrinted edition: _z9781493944668
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1059-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2014
999			_c234747 _d234747