000			05012nam a22003615i 4500
001			234753
003			ES-VaUE
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007			cr nn 008mamaa
008			100318s2010 xxu| s |||| 0|eng d
020			_a9781607616115
024	7		_a10.1007/978-1-60761-611-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMolecular and Cell Biology Methods for Fungi _cedited by Amir Sharon.
250			_a1st edition 2010
264		1	_aTotowa, NJ _bHumana Press _c2010
300			_a1 recurso en línea (XI, 321 páginas) _b53 ilustraciones, 1 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v638
505	0		_aFungal Transformation and Gene Knockout -- Protoplast Transformation of Filamentous Fungi -- Electroporation and Agrobacterium-Mediated Spore Transformation -- High-Throughput Construction of Gene Deletion Cassettes for Generation of Neurospora crassa Knockout Strains -- Development of Impala-Based Transposon Systems for Gene Tagging in Filamentous Fungi -- DelsGate: A Robust and Rapid Method for Gene Deletion -- Gene Silencing for Functional Analysis: Assessing RNAi as a Tool for Manipulation of Gene Expression -- Detection and Quantification of Fungi -- Analysis of Fungal Gene Expression by Real Time Quantitative PCR -- Identification of Differentially Expressed Fungal Genes In Planta by Suppression Subtraction Hybridization -- Quantification of Fungal Infection of Leaves with Digital Images and Scion Image Software -- Expression Profiling of Fungal Genes During Arbuscular Mycorrhiza Symbiosis Establishment Using Direct Fluorescent In Situ RT-PCR -- Application of Laser Microdissection to Study Plant-Fungal Pathogen Interactions -- Multiplex Gene Expression Analysis by TRAC in Fungal Cultures -- Amplification of Fungal Genomes Using Multiple Displacement Amplification -- Microscopy and Protein Analysis -- Biochemical Methods Used to Study the Gene Expression and Protein Complexes in the Filamentous Fungus Neurospora crassa -- Measuring Protein Kinase and Sugar Kinase Activity in Plant Pathogenic Fusarium Species -- A Detailed Protocol for Chromatin Immunoprecipitation in the Yeast Saccharomyces cerevisiae -- A Method to Visualize the Actin and Microtubule Cytoskeleton by Indirect Immunofluorescence -- Fluorescence In Situ Hybridization for Molecular Cytogenetic Analysis in Filamentous Fungi -- Live-Cell Imaging of Microtubule Dynamics in Hyphae of Neurospora crassa -- Methods to Detect Apoptotic-Like Cell Death in Filamentous Fungi -- Evaluation of Antifungal Susceptibility Using Flow Cytometry -- Preparation of Fungi for Ultrastructural Investigations and Immunogoldlabelling -- Split-EGFP Screens for the Detection and Localisation of Protein-Protein Interactions in Living Yeast Cells.
520			_aWith the development of fungal transformation systems and the deciphering of an increasing number of fungal genomes, this diverse clade of heterotrophic eukaryotic organisms has proven to be ideal for molecular work and highly serviceable as model systems to study basic processes with results that are applicable to many organisms, including humans. In Molecular and Cell Biology Methods for Fungi, experts in the field provide an up-to-date set of practical protocols covering a range of frequently used methods used to study molecular and cellular aspects of fungal biology. The included classical protocols such as transformation systems and traditional protein analysis methods, which have been widely used for many years, alongside the most advanced techniques such as genome amplification, whole genome knockout methods, and sophisticated in vivo imaging techniques will prove to be easily adaptable and useful in a wide range of species. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Molecular and Cell Biology Methods for Fungi seeks to provide cutting-edge techniques in order to aid both experienced fungal research laboratories as well as those that are interested in using fungi as hosts to study their favorite genes.
700	1		_aSharon, Amir _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781607616146
776	0	8	_iPrinted edition: _z9781607616108
776	0	8	_iPrinted edition: _z9781493961467
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-611-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2010
999			_c234753 _d234753