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001			234762
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007			cr nn 008mamaa
008			120914s2012 xxu| s |||| 0|eng d
020			_a9781627030021
024	7		_a10.1007/978-1-62703-002-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aNanotoxicity _bMethods and Protocols _cedited by Joshua Reineke.
250			_a1st edition 2012
264		1	_aTotowa, NJ _bHumana Press _c2012
300			_a1 recurso en línea (XII, 414 páginas) _b85 ilustraciones, 47 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v926
505	0		_aHistorical Overview of Nanotechnology and Nanotoxicology -- Characterization of Nanomaterials for Toxicological Studies -- Methods for Understanding the Interaction Between Nanoparticles and Cells -- Single-Cell Gel Electrophoresis (COMET) Assay in Nano-Genotoxicology -- Single-Cell Nanotoxicity Assays of Superparamagnetic Iron Oxide Nanoparticles (SPIONs) -- Western Blot Analysis -- Application of Reverse Transcription-PCR and Real-Time PCR in Nanotoxicity Research -- Deriving TC50 Values of Nanoparticles from Electrochemical Monitoring of Lactate Dehydrogenase (LDH) Activity Indirectly -- Enzyme-Linked Immunosorbant Assay of IL-8 Production in Response to Silver Nanoparticles -- Metabolomics Techniques in Nanotoxicology Studies -- Nanoparticle Uptake Measured by Flow Cytometry -- Determining Biological Activity of Nanoparticles as Measured by Flow Cytometry -- Whole Cell Impedance Biosensoring Devices -- Free Energy Calculation of Permeant-Membrane Interactions Using Molecular Dynamics Simulations -- Screening of Fullerene Toxicity by Haemolysis Assay -- Assessment of In Vitro Skin Irritation Potential of Nanoparticles: RHE Model -- In Vivo Methods of Nanotoxicology -- The Luminescent Bacteria Test to Determine the Acute Toxicity of Nanoparticle Suspensions -- The Primacy of Physicochemical Characterization of Nanomaterials for Reliable Toxicity Assessment: A Review of the Zebrafish Nanotoxicology Model -- Application of Embryonic and Adult Zebrafish for Nanotoxicity Assessment -- Applications of Subsurface Microscopy -- Application of ICP-MS for the Study of Disposition and Toxicity of Metal-Based Nanomaterials -- Quantitative Nanoparticle Organ Disposition by Gel Permeation Chromatography -- Physiologically-Based Pharmacokinetic Modeling For Nanoparticle Toxicity Study -- Biophysical Methods for Assessing Plant Responses to Nanoparticle Exposure -- In Vivo Nanotoxicity Assays in Plant Models.
520			_aThe field of nanotechnology has developed very rapidly over the past decade lending great promise to medical applications in drug delivery, therapeutics, and biological imaging. Due to the great promise, rapid development, and broad application of nanomaterials, it is imperative that researchers from development through application seek a thorough understanding of nanotoxicity.  Nanotoxicity: Methods and Protocols address the special considerations when applying toxicity studies to nanomaterials and detail newly developed methods for the study of nanotoxicity.  These diverse methods span in vitro cell culture, model tissues, in situ exposure, in vivo models, analysis in plants, and mathematical modeling, proving to be relevant to pharmaceutical scientists, material scientists, bioengineers, toxicologists, environmentalists, immunologists, and cellular and molecular biologists, to name a few.  As part of the highly successful Methods in Molecular Biology™, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Comprehensive and cutting-edge, Nanotoxicity: Methods and Protocols aims to diversify the capabilities of current researchers involved in nanotoxicology and to enable researchers in related fields to expand their knowledge of how nanomaterials interface with the biological environment.
700	1		_aReineke, Joshua _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627030014
776	0	8	_iPrinted edition: _z9781627030038
776	0	8	_iPrinted edition: _z9781493958757
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-002-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2012
999			_c234762 _d234762