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001			234764
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007			cr nn 008mamaa
008			150930s2015 xxu| s |||| 0|eng d
020			_a9781493928422
024	7		_a10.1007/978-1-4939-2842-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aC. elegans _bMethods and Applications _cedited by David Biron, Gal Haspel.
250			_a2nd edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XII, 252 páginas) _b57 ilustraciones, 44 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1327
505	0		_aLibrary Construction for Mutation Identification by Whole-Genome Sequencing -- Fundamentals of Comparative Genome Analysis in Caenorhabditis Nematodes -- Genetic Methods for Cellular Manipulations in C. elegans -- A Fusion PCR Method for Expressing Genetic Tools in C. elegans -- Transposon Assisted Genetic Engineering with Mos1 Mediated Single-copy Insertion (MosSCI) -- Creating Genome Modifications in C. elegans using the CRISPR/Cas9 System -- Observing and Quantifying Fluorescent Reporters -- Microbial Rhodopsin Optogenetic Tools: Application for Analyses of Synaptic Transmission and of Neuronal Network Activity in Behavior -- Simultaneous Optogenetic Stimulation of Individual Pharyngeal Neurons and Monitoring of Feeding Behavior in Intact C. elegans -- High-Pressure Freeze and Freeze Substitution Electron Microscopy in C. elegans -- Electron Tomography Methods for C. elegans -- Microfluidic Devices for Behavioral Analysis, Microscopy, and Neuronal Imaging in Caenorhabditis elegans -- Tracking Single C. elegans using a USB Microscope on a Motorized Stage -- An Imaging System for C. elegans Behavior -- A Method for Obtaining Large Populations of Synchronized Caenorhabditis elegans dauer Larvae -- Sampling and Isolation of C. elegans from the Natural Habitat -- A Primer on Prototyping -- A Primer on Quantitative Modeling.
520			_aThe aim of this volume is to provide a step-by-step guide for implementing a selection of novel techniques in the lab. Each protocol in this volume is presented as a standalone chapter, specifically geared towards addressing practical needs without presuming prior knowledge of the technique at hand. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, C. elegans: Methods and Applications, Second Edition aims to ensure successful results in the further study of this dynamic field.
700	1		_aBiron, David _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aHaspel, Gal _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493928415
776	0	8	_iPrinted edition: _z9781493928439
776	0	8	_iPrinted edition: _z9781493948925
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2842-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c234764 _d234764