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001			234765
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008			100301s2005 xxu| s |||| 0|eng d
020			_a9781592598694
024	7		_a10.1385/1592598692 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aChemosensitivity _bVolume I: In Vitro Assays _cedited by Rosalyn D. Blumenthal.
250			_a1st edition 2005
264		1	_aTotowa, NJ _bHumana Press _c2005
300			_a1 recurso en línea (XIV, 231 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v110
505	0		_aAn Overview of Chemosensitivity Testing -- Clonogenic Cell Survival Assay -- High-Sensitivity Cytotoxicity Assays for Nonadherent Cells -- Sulforhodamine B Assay and Chemosensitivity -- Use of the Differential Staining Cytotoxicity Assay to Predict Chemosensitivity -- Collagen Gel Droplet Culture Method to Examine In Vitro Chemosensitivity -- The MTT Assay to Evaluate Chemosensitivity -- Histoculture Drug Response Assay to Monitor Chemoresponse -- In Vitro Testing of Chemosensitivity in Physiological Hypoxia -- Chemosensitivity Testing Using Microplate Adenosine Triphosphate-Based Luminescence Measurements -- High-Throughput Technology -- Dimscan -- The ChemoFx® Assay -- Evaluating Response to Antineoplastic Drug Combinations in Tissue Culture Models -- Image Analysis Using the Fluorochromasia Assay to Quantify Tumor Drug Sensitivity -- Immunohistochemical Detection of Ornithine Decarboxylase as a Measure of Chemosensitivity Testing -- Immunohistochemistry of p53, Bcl-2, and Ki-67 as Predictors of Chemosensitivity.
520			_aChemosensitivity testing is an ex vivo means of determining or enhancing the cytotoxic and/or cytostatic, or apoptosis-inducing effects of anticancer drugs. In Chemosensitivity, leading researchers and physicians working in academia and biotech companies describe state-of-the-art laboratory methods for assessing chemosensitivity in vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in individual tumors. Volume 1: In Vitro Assays provides a panel of 16 in vitro measures of chemosensitivity in adherent and nonadherent cells for single agents and combinations of agents. In addition to immunohistochemical and imaging approaches, these assays include clonogenic, colorimetric, fluorometric, and physiological assays. Highlights include image analysis to assess drug sensitivity, high throughput approaches using green fluorescent protein, DIMSCAN (a microcomputer fluorescence-based assay), and the ChemoFx assay used in biotechnology. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. The authors also provide guidance on how best to analyze the data derived from the protocols. A companion volume, Volume 2: In Vivo Models, Imaging, and Molecular Regulators, provides protocols for classifying tumors into response categories and customizing chemotherapy regimens to individual patients. Cutting-edge and highly practical, the two volumes of Chemosensitivity provide a comprehensive collection of readily reproducible techniques for the in vitro and in vivo screening of new agents and a set of proven approaches to understand mechanistically why certain cancer cell lines (in vitro) of tumors (in vivo) are more, or less, sensitive to a particular agent.
700	1		_aBlumenthal, Rosalyn D _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617375088
776	0	8	_iPrinted edition: _z9781588293459
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592598692 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2005
999			_c234765 _d234765