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020 _a9781597450621
024 7 _a10.1007/978-1-59745-062-1
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aPlant Pathology
_bTechniques and Protocols
_cedited by Robert Burns.
250 _a1st edition 2009
264 1 _aTotowa, NJ
_bHumana Press
_c2009
300 _a1 recurso en línea (X, 326 páginas)
_b42 ilustraciones
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v508
505 0 _aBioassay for Diagnosis of Plant Viruses -- Development of Enzyme Linked, Tissue Blot and Dot Blot Immunoassays for Plant Virus Detection -- Immunisation strategies for Antibody Production -- Preparation of Immunogens and Production of Antibodies -- Enzyme-Linked Immunosorbent Assay Detection of Ralstonia solanacearum in Potatoes: The South African Experience -- Production of Monoclonal Antibodies to Plant Pathogens -- Enzyme-Linked Immunosorbent Assay for the Detection and Identification of Plant Pathogenic Bacteria (In Particular for Erwinia amylovora and Clavibacter michiganensis subsp. sepedonicus) -- Indirect Immunofluorescence Microscopy for the Detection and Identification of Plant Pathogenic Bacteria (In Particular for Ralstonia solanacearum) -- Detection of Four Major Bacterial Potato Pathogens -- Erwinia amylovora: Modern Methods for Detection and Differentiation -- The use of Fluorescent in situ Hybridisation in Plant Fungal Identification and Genotyping -- Use of Molecular Methods for the Detection of Fungal Spores -- Identification of Phytophthora fragariae var. rubi by PCR -- Detection of Double-Stranded RNA Elements in the Plant Pathogenic Fungus Rhizoctonia solani -- Immunocapture-PCR for Plant Virus Detection -- Multiplex Polymerase Chain Reaction (PCR) and Real-time Multiplex PCR for the Simultaneous Detection of Plant Viruses -- Fluorescent-based Techniques for Viral Detection, Quantification andCharacterisation -- Analysis of Population Structures of Viral Isolates using Single StrandConformation Polymorphism (SSCP) Method -- Direct Detection of Plant Viruses in Potato Tubers using Real-time PCR -- An Accelerated Soil Bait Assay for the Detection of Potato Mop Top Virus in Agricultural Soil -- Detection of Phytoplasmas of Temperate Fruit Trees -- PCR Detection of Potato Cyst Nematode -- Potato Cultivar Genome Analysis -- Barley Variety Identification Using SSRs.
520 _aPlant diseases can have an enormous impact on our lives. In a world where total crop failure can quickly lead to human misery and starvation, accurate diagnostics play a key role in keeping plants free from pathogens. In Plant Pathology: Techniques and Protocols, expert researchers provide methods which are vital to the diagnosis of plant diseases across the globe, addressing all three categories of plant pathology techniques: traditional, serological, and nucleic acid. Chapters examine recent and developing issues with crop identity and authenticity, allowing workers to genotype samples from two major food groups. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Authoritative and reader-friendly, Plant Pathology: Techniques and Protocols is an incredible guide which will soon prove to be indispensable, both to novices and expert researchers alike. .
700 1 _aBurns, Robert
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781607610939
776 0 8 _iPrinted edition:
_z9781617377853
776 0 8 _iPrinted edition:
_z9781588297990
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-062-1
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2009
999 _c234768
_d234768