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020 _a9781493970216
024 7 _a10.1007/978-1-4939-7021-6
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _a3D Cell Culture
_bMethods and Protocols
_cedited by Zuzana Koledova.
250 _a1st edition 2017
264 1 _aNew York, NY
_bSpringer International Publishing
_c2017
300 _a1 recurso en línea (XVI, 452 páginas)
_b114 ilustraciones, 99 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1612
505 0 _a3D Cell Culture: An Introduction -- Preparation of Decellularized Biological Scaffolds for 3D Cell Culture -- 3D Cell Culture in Interpenetrating Networks of Alginate and rBM Matrix -- Hydrogel-Based In Vitro Models of Tumor Angiogenesis -- Generation of Induced Pluripotent Stem Cells in Defined Three-Dimensional PEG Hydrogels -- Calcium Phosphate Foams: Potential Scaffolds for Bone Tissue Modelling in Three-Dimensions.oids -- 3d="" fluid-dynamic="" models="" multiple="" myeloma -- preparation="" three-dimensional="" full="" thickness="" skin="" equivalent -- analysis="" breast="" cell="" invasion="" using="" an="" system -- 3d="" the="" brain="" parenchyma="" metastasis="" interface="" metastasis -- 3d="" neural="" in="" dual="" hydrogel="" systems -- 3d="" micro-patterned="" hydrogels="" prepared="" by="" photomask,="" micro-needle,="" or="" soft="" lithography="" techniques -- 3d="" niche="" engineering="" via="" two-photon="" laser="" polymerization -- microfluidic-based="" generation="" collagen="" spheres="" investigate="" multicellular="" spheroid="" invasion -- high-throughput="" sphere="" formation="" culture -- high-throughput="" tumor="" recyclable="" microfluidic="" platform -- high-throughput="" platform="" mesenchymal="" anastomosed="" microvascular="" network="" living="" capillary="" networks="" and="" endothelial="" cell-lined="" channels -- human="" small="" airway-on-a-chip="" protocol -- microfluidic="" bioprinting="" heterogeneous="" tissue="" constructs -- bioprinting="" decellularized="" extracellular="" matrix="" bioink -- bioprinting="" cartilage="" peg="" hydrogel -- real-time="" cycle="" imaging="" melanoma -- revealing="" ultrastructure="" morphology="" spheroids="" electron="" microscopy -- quantitative="" phenotypic="" image="" analysis="" cultures.
520 _aThis book provides an overview of established 3D cell culture assays from leaders in the field. Their contributions cover a wide spectrum of techniques and approaches for 3D cell culture, from organoid cultures through organotypic models to microfluidic approaches and emerging 3D bioprinting techniques, which are used in developmental, stem cell, cancer, and pharmacological studies, among many others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, 3D Cell Culture: Methods and Protocols aims to inspire researchers to develop novel 3D cell culture techniques according to their specific scientific needs and interests, leading to a new generation of physiologically relevant and realistic 3D cell cultures. Chapter 15 of this book is available open access under a CC BY 4.0 license.
700 1 _aKoledova, Zuzana
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493970193
776 0 8 _iPrinted edition:
_z9781493970209
776 0 8 _iPrinted edition:
_z9781493983704
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7021-6
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2017
999 _c234776
_d234776