000			03057nam a22003495i 4500
001			234791
003			ES-VaUE
005			20221220020625.0
007			cr nn 008mamaa
008			100301s2007 xxu| s |||| 0|eng d
020			_a9781597453776
024	7		_a10.1385/1597453773 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPyrosequencing Protocols _cedited by Sharon Marsh.
250			_a1st edition 2007
264		1	_aTotowa, NJ _bHumana Press _c2007
300			_a1 recurso en línea (XII, 196 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v373
505	0		_aThe History of Pyrosequencing® -- Pyrosequencing® Applications -- Web-Based Primer Design Software for Genome-Scale Genotyping by Pyrosequencing® -- Pyrosequencing® -- Universal Primer Applications for Pyrosequencing® -- Allele Quantification and DNA Pooling Methods -- Multiplex Pyrosequencing® for DNA Variation Analysis -- Analysis of Gene-Specific DNA Methylation Patterns by Pyrosequencing® Technology -- Detecting Mutations That Confer Oxazolidinone Resistance in Gram-Positive Bacteria -- Pyrosequencing®-Based Strategies for Improved Allele Typing of Human Leukocyte Antigen Loci -- Pyrosequencing® of Phage Display Libraries for the Identification of Cell-Specific Targeting Ligands -- Gene Copy Number Detection in Animal Studies -- Detection of Allelic Imbalance in Gene Expression Using Pyrosequencing® -- Pyrosequencing® Genotype Storage Techniques.
520			_aPyrosequencing® Protocols presents detailed protocols for the multidisciplinary application of Pyrosequencing® technology, all written by world-renowned experts in the field. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing®, and supplementing each protocol with troubleshooting tips specific to that method. Chapters 1 and 2 present an introductory overview of the origins of the Pyrosequencing® methodology and its key applications. Chapters 3 and 4 describe primer selection and the basic Pyrosequencing® technique, and Chapters 5-7 provide methods for improving throughput and decreasing cost. Detailed applications for Pyrosequencing® comprise Chapters 8-13, and the important aspects of data storage is discussed in Chapter 14. This comprehensive volume both highlights the versatility of and provides detailed protocols for the application of Pyrosequencing®.
700	1		_aMarsh, Sharon _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617376931
776	0	8	_iPrinted edition: _z9781588296450
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1597453773 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2007
999			_c234791 _d234791