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001			234795
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007			cr nn 008mamaa
008			170612s2017 xxu| s |||| 0|eng d
020			_a9781493971114
024	7		_a10.1007/978-1-4939-7111-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aZymography _bMethods and Protocols _cedited by Jeff Wilkesman, Liliana Kurz.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XX, 310 páginas) _b67 ilustraciones, 45 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1626
505	0		_aZymography Principles -- Serine Protease Zymography: Low-Cost, Rapid, and Highly Sensitive RAMA Casein Zymography -- Cysteine Protease Zymography: Brief Review -- Aspartic Protease Zymography Case Study: Detection of Fungal Acid Proteases by Zymography -- Detection of Aspartic Proteinase Activities Using Gel Zymography -- MMP Activity Detection in Zymograms -- Characterization of Novel Collagenolytic Proteases -- Zymography as a Research Tool in the Study of Matrix Metalloproteinase Inhibitors -- Detection and Characterization of Bacterial Proteinases Using Zymography -- A Sensitive, Rapid, and Specific Technique for the Detection of Collagenase Using Zymography -- Reverse Zymography: Overview and Pitfalls -- Cell In Situ Zymography: Imaging Enzyme-Substrate Interactions -- Examination of Gelatinase Isoforms in Rodent Models of Acute Neurodegenerative Diseases Using Two-Dimensional Zymography -- Two-Dimensional Zymography of Proteases from Steatotic Duck Liver -- Simultaneous Detection of Activity and Relative Molecular Mass of Adenylate Kinases after SDS-PAGE and Blotting -- Silver-Stained Fibrin Zymography: Separation of Proteases and Activity Detection Using a Single Substrate-Containing Gel -- Zymography Methods to Simultaneously Analyze Superoxide Dismutase and Catalase Activities: Novel Application for Yeast Species Identification -- Detection of Guaiacol Peroxidase on Electrophoretic Gels -- In Situ Demonstration and Characteristic Analysis of the Protease Using Substrate Immersing Zymography -- Use of Zymography in Trypanosomiasis Studies -- Zymography in Multiwells for Quality Assessment of Proteinases -- Visualization of Enzyme Activities in Earthworm Biopores by In Situ Soil Zymography -- Multiplex Cathepsin Zymography to Detect Amounts of Active Cathepsins K, L, S, and V -- Transfer Zymography -- Sequential Detection of Thermophilic Lipase and Protease by Zymography -- Calpain Zymography: General Methodology and Protocol -- CTAB Zymography for the Analysis of Aspartic Proteases from Marine Sponges -- Zymography Detection of a Bacterial Extracellular Thermoalkaline Esterase/Lipase Activity -- Amylase Zymography.
520			_aThis detailed volume examines the latest techniques and protocols associated with zymography, the practice of detecting enzyme activity on electrophoretic gels. Sections cover subjects such as endopeptidase zymography, reverse zymography & in situ zymography, 2D zymography, as well as a variety of special cases. Written in the highly respected Methods in Molecular Biology series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Zymography: Methods and Protocols serves as an ideal aid for a broad array of researchers from the fields of biological and medicinal science, as well as scientists working in clinical and diagnostic medicine, medical genetics, agricultural entomology, genetic monitoring of environmental pollution, and forensic science.
700	1		_aWilkesman, Jeff _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aKurz, Liliana _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493971091
776	0	8	_iPrinted edition: _z9781493971107
776	0	8	_iPrinted edition: _z9781493983872
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7111-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c234795 _d234795