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001			234796
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008			100721s2010 xxu| s |||| 0|eng d
020			_a9781597453646
024	7		_a10.1007/978-1-59745-364-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aCyclooxygenases _bMethods and Protocols _cedited by Samir S. Ayoub, Roderick J. Flower, Michael Seed.
250			_a1st edition 2010
264		1	_aTotowa, NJ _bHumana Press _c2010
300			_a1 recurso en línea (X, 218 páginas) _b38 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v644
505	0		_aIn Vitro Protocols for Studying Expression and Activity of Cyclooxygenase Enzymes and Characterisation of Their Inhibition Patterns -- In Vitro Cyclo-oxygenase Expression and Activity Protocols: Introduction to Part I -- Techniques Used to Study Regulation of Cyclooxygenase-2 Promoter Sites -- Purification of Recombinant Human COX-1 and COX-2 -- Cloning and Expression of Cyclooxygenase-1 and Cyclooxygenase-2 -- Expression of Cyclooxygenase Isoforms in the Baculovirus Expression System -- Peroxidase Active Site Activity Assay -- Study of Inhibitors of the PGH Synthases for which Potency Is Regulated by the Redox State of the Enzymes -- Different Methods for Testing Potential Cyclooxygenase-1 and Cyclooxygenase-2 Inhibitors -- In Vitro Cyclooxygenase Activity Assay in Tissue Homogenates -- Techniques Used to Characterize the Binding of Cyclooxygenase Inhibitors to the Cyclooxygenase Active Site -- In Vitro Assays for Cyclooxygenase Activity and Inhibitor Characterization -- Extraction and Measurement of Prostanoids and Isoprostanes -- Extraction and Measurement of Prostanoids and Isoprostanes: Introduction to Part II -- Prostanoid Extraction and Measurement -- Measurement of 8-Iso-Prostaglandin F2? in Biological Fluids as a Measure of Lipid Peroxidation -- In Vivo Models to Study Involvement of Cyclooxygenase Products in Health and Disease -- In Vivo Models to Study Cyclooxygenase Products in Health and Disease: Introduction to Part III -- Protocols to Assess the Gastrointestinal Side Effects Resulting from Inhibition of Cyclo-Oxygenase Isoforms -- Cyclooxygenase Enzymes and Their Products in the Carrageenan-Induced Pleurisy in Rats -- Iloprost-Induced Nociception: Determination of the Site of Anti-nociceptive Action of Cyclooxygenase Inhibitors and the Involvement of Cyclooxygenase Products in Central Mechanisms of Nociception.
520			_aSince the discovery of the pharmacological and toxicological importance of inhibiting the cyclooxygenase (COX) enzymes by non-steroidal anti-inflammatory drugs (NSAIDs), much research has gone into the development of methods to study the biological functions of COX-1 and COX-2. In Cyclooxygenases: Methods and Protocols, experts and pioneers in the field present the most up-to-date in vitro and in vivo techniques routinely used in COX research. The volume delves into essential topics such as the purification, cloning, and expression of COX enzymes as well as in vitro assays aimed at determining the inhibitory potency of drugs on COX-1 and COX-2 activities, with chapters describing protocols used for the extraction and measurement of the prostanoids. This volume also describes in vivo disease models used to study the roles of COX-1 and COX-2 in gastrointestinal injury, inflammation, and pain. As a book in the highly successful Methods in Molecular Biology™ series, the protocols chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cyclooxygenases: Methods and Protocols serves as an indispensable tool for all scientists seeking the treatment of inflammation, pain, fever, and other harmful conditions.
700	1		_aAyoub, Samir S _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aFlower, Roderick J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aSeed, Michael _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781607616931
776	0	8	_iPrinted edition: _z9781588299536
776	0	8	_iPrinted edition: _z9781493956388
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-364-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2010
999			_c234796 _d234796