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020 _a9781493916801
024 7 _a10.1007/978-1-4939-1680-1
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 4 _aThe Nucleus
_cedited by Ronald Hancock.
250 _a2nd edition 2015
264 1 _aNew York, NY
_bSpringer International Publishing
_c2015
300 _a1 recurso en línea (XI, 237 páginas)
_b54 ilustraciones, 35 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1228
505 0 _aCell Type-Specific Affinity Purification of Nuclei for Chromatin Profiling in Whole Animals -- Lysis Gradient Centrifugation - A Flexible Method for the Isolation of Nuclei from Primary Cells -- Isolation of Nuclei in Media Containing an Inert Polymer to Mimic the Crowded Cytoplasm -- A New Rapid Method for Isolating Nucleoli -- Sequential Recovery of Macromolecular Components of the Nucleolus -- Au Nanoinjectors for Electrotriggered Gene Delivery into the Cell Nucleus -- Improving Chromatin Immunoprecipitation (ChIP) by Suppression of Method-Induced DNA-Damage Signaling -- Purification of Specific Chromatin Loci for Proteomic Analysis -- Chromatin Structure Analysis of Single Gene Molecules by Psoralen Crosslinking and Electron Microscopy -- Purification of Proteins on Newly Synthesized DNA Using iPOND -- Applying the Ribopuromycylation Method to Detect Nuclear Translation -- Targeted Nano Analysis of Water and Ions in the Nucleus Using Cryo-Correlative Microscopy -- A Redox-Sensitive Yellow Fluorescent Protein Sensor for Monitoring Nuclear Glutathione Redox  Dynamics -- Determination of the Dissociation Constant of the NFkB p50/p65 Heterodimer in Living Cells Using Fluorescence Cross-Correlation Spectroscopy -- Imaging and Quantification of Amyloid Fibrillation in the Cell Nucleus -- Analysis of Nuclear Organization with TANGO, Software for High-Throughput Quantitative Analysis of 3D Fluorescence Microscopy Images -- Quantitative Analysis of Chromosome Localization in the Nucleus.
520 _aThis volume presents detailed, recently-developed protocols ranging from isolation of nuclei to purification of chromatin regions containing single genes, with a particular focus on some less well-explored aspects of the nucleus. The methods described include new strategies for isolation of nuclei, for purification of cell type-specific nuclei from a mixture, and for rapid isolation and fractionation of nucleoli. For gene delivery into and expression in nuclei, a novel gentle approach using gold nanowires is presented. As the concentration and localization of water and ions are crucial for macromolecular interactions in the nucleus, a new approach to measure these parameters by correlative optical and cryo-electron microscopy is described. The Nucleus, Second Edition presents methods and software for high-throughput quantitative analysis of 3D fluorescence microscopy images, for quantification of the formation of amyloid fibrils in the nucleus, and for quantitative analysis of chromosome territory localization. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, The Nucleus, Second Edition seeks to serve both professionals and novices with its well-honed methods for the study of the nucleus.
700 1 _aHancock, Ronald
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493916795
776 0 8 _iPrinted edition:
_z9781493916818
776 0 8 _iPrinted edition:
_z9781493948734
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1680-1
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2015
999 _c234809
_d234809