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008			161110s2017 xxu| s |||| 0|eng d
020			_a9781493965182
024	7		_a10.1007/978-1-4939-6518-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aEukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation _cedited by Narendra Wajapeyee, Romi Gupta.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 278 páginas) _b49 ilustraciones, 29 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1507
505	0		_aFluorescence Reporter-Based Genome-Wide RNA Interference Screening to Identify Alternative Splicing Regulators -- Tandem Affinity Purification Approach Coupled To Mass Spectrometry to Identify Post-translational Modifications of Histones Associated with Chromatin-binding Proteins -- Efficient Preparation of High-Complexity ChIP-Seq Profiles from Early Xenopus Embryos -- Systematic Discovery Of Chromatin-bound Protein Complexes from ChIP-seq Datasets -- Assay for Transposase-Accessible Chromatin with high throughput sequencing (ATAC-seq) Protocol for Zebrafish Embryos -- Establishment of time- and cell-specific RNAi in Caenorhabditis elegans -- Cell-penetrating Peptide-mediated Delivery of Cas9 Protein and Guide RNA for Genome Editing -- Epigenetic Analysis of Endocrine Cell Subtypes from Human Pancreatic Islets -- eIF3 Regulation of Protein Synthesis, Tumorigenesis, and Therapeutic Response -- High Resolution Gene Expression Profiling of RNA Synthesis, Processing, and Decay by Metabolic Labeling of Newly Transcribed RNA using 4-thiouridine -- Accurate Detection of Differential Expression and Splicing using Low-level Features -- Profiling Changes in Histone Post-translational Modifications by Top-Down Mass Spectrometry -- Determining if an mRNA is a Substrate of Nonsense-mediated mRNA Decay in Saccharomyces cerevisiae -- Optimizing In Vitro Pre-mRNA 3' Cleavage Efficiency: Reconstitution from Anion-Exchange Separated HeLa Cleavage Factors and from Adherent HeLa Cell Nuclear Extract -- Unbiased Interrogation of 3D Genome Topology using Chromosome Conformation Capture Coupled to High-throughput Sequencing (3C-Seq) -- Using an Inducible CRISPR-Cas9-KRAB Effector System to Dissect Transcriptional Regulation in Human Embryonic Stem Cells -- In vitro assay to Study Histone Ubiquitination during Transcriptional Regulation -- Determination of Alternate Splicing Events using Transcriptome Arrays -- Large-scale RNA Interference Screening to Identify Transcriptional Regulators of a Tumor Suppressor -- GeneTranscriptional Analysis-based Integrative Genomics Approach to Identify Tumor Promoting Metabolic Genes. .
520			_a This volume describes a variety of protocols that will allow the readers to study different aspects of transcriptional and posttranscriptional gene expression regulation in eukaryotic cells. Chapters focus on the latest use of CRISPRi and RNAi technologies for studying various aspects of transcriptional and posttranscriptional regulation and tools to navigate protocols on key bioinformatics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls Authoritative and cutting-edge, Eukaryotic Transcription and Post-Transcription Gene Expression Regulation aims to ensure successful results in the further study of this vital field.
700	1		_aWajapeyee, Narendra _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aGupta, Romi _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493965168
776	0	8	_iPrinted edition: _z9781493965175
776	0	8	_iPrinted edition: _z9781493982233
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6518-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c234818 _d234818