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020 _a9783662491409
024 7 _a10.1007/978-3-662-49140-9
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aHydrocarbon and Lipid Microbiology Protocols
_bActivities and Phenotypes
_cedited by Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales.
250 _a1st edition 2017
264 1 _aBerlin, Heidelberg
_bSpringer International Publising
_c2017
300 _a1 recurso en línea (X, 244 páginas)
_b40 ilustraciones, 20 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aSpringer Protocols Handbooks
_x1949-2456
505 0 _aIntroduction -- Protocols for the measurement of bacterial chemotaxis to hydrocarbons -- Determining the tendency of microorganisms to interact with hydrocarbon phases -- Protocols for measuring biosurfactant production in microbial cultures -- Protocol for the measurement of hydrocarbon transport in bacteria -- Bacterial solvent response and tolerance: cis-trans isomerization -- Analysis of PHB metabolism applying Tn5 mutagenesis in Ralstonia eutropha -- Phenotyping microarrays for the characterization of environmental microorganisms -- Control of the concentration of aquatic hydrocarbons by microorganisms -- Protocols for measuring methanogenesis -- Respiration rate determined by phosphorescence-based sensors -- Measuring the impact of hydrocarbons on rates of nitrogen fixation -- Protocols for measuring activity of sulphate-reducing bacteria in water injection systems -- Protocol for evaluating the biological stability of fuel formulations and their relationship to carbon steel biocorrosion -- Laboratory protocols for investigating microbial souring and potential treatments in crude oil reservoirs.
520 _aThis Volume features protocols for investigating the hydrocarbon- and lipid-specific activities of microbes. They include methods for studying chemotaxis, the colonisation of hydrocarbon surfaces, hydrocarbon uptake, respiration, nitrogen fixation, sulphate reduction, membrane stabilisation through cis-trans isomerisation of membrane fatty acids, and the production of biosurfactants and biopolymers in response to the presence of hydrocarbons. A protocol for studying the ability of microbes to control the concentration of hydrocarbons in their aqueous environment is also described, and phenotyping methods to reveal microbes' more general metabolic activities are presented. Several protocols for investigating acid production in connection with oil souring and biocorrosion by microbes in oil well, oil transportation and storage settings are presented. Lastly, protocols for measuring methanogenesis, as an example of microbial hydrocarbon production, are described. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
700 1 _aMcGenity, Terry J
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aTimmis, Kenneth N
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aNogales, Balbina
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9783662491386
776 0 8 _iPrinted edition:
_z9783662491393
776 0 8 _iPrinted edition:
_z9783662569856
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-3-662-49140-9
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2017
999 _c234837
_d234837