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020 _a9781597452045
024 7 _a10.1007/978-1-59745-204-5
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aHost-Pathogen Interactions
_bMethods and Protocols
_cedited by Steffen Rupp, Kai Sohn.
250 _a1st edition 2009
264 1 _aTotowa, NJ
_bHumana Press
_c2009
300 _a1 recurso en línea (XII, 428 páginas)
_b86 ilustraciones, 2 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v470
505 0 _aBacterial Pathogens -- Introduction: Bacterial Pathogens -- Transcriptome Analyses in the Interaction of Neisseria meningitidis with Mammalian Host Cells -- Surface-Exposed Adherence Molecules of Streptococcus pneumoniae -- Screening of Virulence Traits in Legionella pneumophila and Analysis of the Host Susceptibility to Infection by Using the Dictyostelium Host Model System -- Characterizing Host Receptor Recognition by Individual Bacterial Pathogens -- Fungal Pathogens -- Introduction: Fungal Pathogens -- Isolation and Purification of Antigenic Components of Cryptococcus -- A Method for Examining Glycans Surface Expression of Yeasts by Flow Cytometry -- Human Epithelial Model Systems for the Study of Candida Infections In Vitro: Part I. Adhesion to Epithelial Models -- Human Epithelial Model Systems for the Study of Candida infections In Vitro: Part II. Histologic Methods for Studying Fungal Invasion -- In Vitro Systems for Studying the Interaction of Fungal Pathogens with Primary Cells from the Mammalian Innate Immune System -- Experimental Infection of Rodent Mammals for Fungal Virulence Testing -- Real-Time and Semiquantitative RT-PCR Methods to Analyze Gene Expression Patterns During Aspergillus-Host Interactions -- Antibody-Based Strategy to Identify Candida albicans Genes Expressed During Infections -- Identification of the Candida albicans Immunome During Systemic Infection by Mass Spectrometry -- Parasites -- Introduction: Parasites -- Axenic In Vitro Cultivation of Echinococcus multilocularis Metacestode Vesicles and the Generationof Primary Cell Cultures -- Culture of Exoerythrocytic Stages of the Malaria Parasites Plasmodium falciparum and Plasmodium vivax -- Modulation of Caspase Activation by Toxoplasma gondii -- Host Responses -- Introduction: Host Responses -- Fungal and Bacterial Killing by Neutrophils -- Endothelial Cell Stimulation by Candida albicans -- Models of Oral and Vaginal Candidiasis Based on In Vitro Reconstituted Human Epithelia for the Study of Host-Pathogen Interactions -- Phagocytosis of Candida albicans by RNAi-Treated Drosophila S2 Cells -- Oral Mucosal Cell Response to Candida albicans in Transgenic Mice Expressing HIV-1 -- Proteomic Profiling of Serologic Response to Candida albicans During Host-Commensal and Host-Pathogen Interactions.
520 _aIn recent decades, infectious diseases, once believed to be fairly contained, have become a vital, resurgent field of research. In Host-Pathogen Interactions: Methods and Protocols, top experts examine the relationship between the host and the pathogen, crucial in the outcome of an infection and the establishment of disease or asymptomatic, commensal colonization by organisms. The step-by-step laboratory methods and protocols of this volume study host-pathogen interaction, with a focus on fungal, bacterial and parasitic pathogens, at a molecular level in order to reveal the mechanisms of infection and to identify the vulnerabilities of the pathogen of interest. Written in the highly successful Methods in Molecular Biology™ series format, the chapters feature brief subject introductions, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Host-Pathogen Interactions: Methods and Protocols serves as an easy entry point for all those investigating the factors responsible for the pathogenicity of microorganisms.
700 1 _aRupp, Steffen
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aSohn, Kai
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781603279895
776 0 8 _iPrinted edition:
_z9781617378249
776 0 8 _iPrinted edition:
_z9781588298867
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-204-5
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2009
999 _c234851
_d234851