000			03687nam a22003495i 4500
001			234858
003			ES-VaUE
005			20221220020629.0
007			cr nn 008mamaa
008			100301s1988 xxu| s |||| 0|eng d
020			_a9781592594917
024	7		_a10.1385/0896031276 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aNew Nucleic Acid Techniques _cedited by John M. Walker.
250			_a1st edition 1988
264		1	_aTotowa, NJ _bHumana Press _c1988
300			_a1 recurso en línea (XVI, 560 páginas) _b17 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v4
505	0		_aElectrophoresis of RNA Denatured with Glyoxal or Formaldehyde -- Northern Blotting -- Hybrid-Release Translation -- Hybrid-Arrested Translation -- In Vitro Translation and Analysis of Early Events in Protein Synthesis Initiation in Nonreticulocyte Mammalian Cells -- Use of Elutips to Purify DNA -- Plasmid Preparation on Sephacryl S1000 -- Electrophoresis of DNA in Nondenaturing Polyacrylamide Gels -- Use of 35S Nucleotides for DNA Sequencing -- Direct Dideoxy DNA Sequencing -- Computer Applications to Molecular Biology -- Detection of Sequence-Specific Protein-DNA Interactions by the DNA-Footprinting Technique -- Oligonucleotide Synthesis Using the Manual Phosphotriester Method -- Manual Oligonucleotide Synthesis Using the Phosphoramidite Method -- Purification of Synthetic Oligonucleotides by Preparative Gel Electrophoresis -- Isolation of High Molecular Weight DNA Suitable for the Construction of Genomic Libraries -- Construction of Mammalian Genomic Libraries Using ? Replacement Vectors -- Cosmid Library Construction -- Construction of cDNA Libraries in ?gt10 or ?gt11 -- Immunoscreening of ?gt11 Expression Libraries Using an Avidin-Biotin Detection System -- Primed Synthesis and Direct Sequencing in the Isolation of cDNA Clones Using Short Oligonucleotides -- Constructing Expression cDNA Libraries Using Unphosphorylated Adaptors -- Expression Screening of cDNA Libraries in pEX -- Producing Antibodies of Predetermined Specificity from Escherichia coli Hybrid Proteins -- Epitope Mapping Using pEX -- DNA Transfection of Mammalian Cells Using Polybrene -- Expression of Foreign Genes in Mammalian Cells -- Dot-Blot Hybridization Method -- Detection of Unique or Low Copy Number DNA Sequences in Complex Genomes -- Photobiotin-Labeled DNA and RNA Hybridization Probes -- Enzyme-Labeled DNA Probes -- Luminescent Detection of Specific DNA Sequences -- Detection of DNA Sequences Using Biotinylated Probes -- Crosslinking of Single-Stranded DNA to Resins -- Extraction of RNA from Plants -- Isolation of Plant Nuclei -- In Vitro Transcription in Plant Nuclei -- Large-Scale Isolation of Ti Plasmid DNA -- Transformation and Regeneration of Dicotyledonous Plants -- Plant Protoplast Fusion -- Plant Tissue Culture -- Direct Gene Transfer into Plant Protoplasts -- Isolation of Wheat Chloroplasts and Chloroplast DNA -- DNA Labeling by Isolated Wheat Chloroplasts.
700	1		_aWalker, John M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489943835
776	0	8	_iPrinted edition: _z9781489943828
776	0	8	_iPrinted edition: _z9780896031272
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896031276 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1988
999			_c234858 _d234858