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008			140416s2014 xxu| s |||| 0|eng d
020			_a9781493907335
024	7		_a10.1007/978-1-4939-0733-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aQuantitative Real-Time PCR _bMethods and Protocols _cedited by Roberto Biassoni, Alessandro Raso.
250			_a1st edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (XI, 231 páginas) _b80 ilustraciones, 66 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1160
505	0		_aTwenty Years of qPCR: A Mature Technology? -- Minimum Information Necessary for Quantitative Real-Time PCR Experiments -- Selection of Reliable Reference Genes for RT-qPCR Analysis -- Introduction to Digital PCR -- mRNA and micro RNA Purity and Integrity: The Key to Success in Expression Profiling -- Mediator Probe PCR: Detection of Real-Time PCR by Label Free Probes and a Universal Fluorogenic Reporter -- Absolute Quantification of Viral DNA: The Quest for Perfection -- A Multiplex Real-Time PCR-Platform Integrated into Automated Extraction Method for the Rapid Detection and Measurement of Oncogenic HPV Type-Specific Viral DNA Load from Cervical Samples -- Real-Time PCR Detection of Mycoplasma pneumoniae in the Diagnosis of  Community-Acquired Pneumonia -- A Sensible Technique to Detect Mollicutes Impurities in Human Cells Cultured in GMP Condition -- Real-Time Quantification Assay to Monitor BCR-ABL1 Transcripts in Chronic Myeloid Leukemia -- A Reliable Assay for Rapidly Defining Transplacental Metastasis Using Quantitative PCR -- Circulating Cell-Free DNA in Cancer -- Gene Expression Analysis by qPCR in Clinical Kidney Transplantation -- Post-Transcriptional Regulatory Networks: From Expression Profiling to Integrative Analysis of mRNA and Micro RNA Data -- Clinical Applications Using Digital PCR -- Developing Non-Invasive Diagnosis for Single Gene Disorders: The Role of Digital PCR.
520			_aQuantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Practical and authoritative, Quantitative Real-Time PCR: Methods and Protocols aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation.
700	1		_aBiassoni, Roberto _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aRaso, Alessandro _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493907342
776	0	8	_iPrinted edition: _z9781493907328
776	0	8	_iPrinted edition: _z9781493946655
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-0733-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2014
999			_c234861 _d234861