000			04320nam a22003615i 4500
001			234863
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007			cr nn 008mamaa
008			130805s2013 xxu| s |||| 0|eng d
020			_a9781627035682
024	7		_a10.1007/978-1-62703-568-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPlant Transposable Elements _bMethods and Protocols _cedited by Thomas Peterson.
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (XV, 324 páginas) _b61 ilustraciones, 23 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1057
505	0		_aHistorical Overview of Transposable Element Research -- Distinguishing Variable Phenotypes from Variegation Caused by Transposon Activities -- Using Transposons for Genetic Mosaic Analysis of Plant Development -- Survey of Natural and Transgenic Gene Markers Used to Monitor Transposon Activity -- Molecular Biology of Maize Ac/Ds Elements - an Overview -- Gene Tagging with Engineered Ds Elements in Maize -- Plant Regeneration Methods for Rapid Generation of a Large Scale of Ds Transposant Population in Rice -- Isolation of Sequences Flanking Ac Insertion Sites by Ac Casting -- Regulation of the Mutator System of Transposons in Maize -- Using MuDR/Mu Transposons in Directed Tagging Strategies -- Genetic and Molecular Analysis of UniformMu Transposon Insertion Lines -- Digestion-Ligation-Amplification (DLA): A Simple Genome Walking Method to Amplify Unknown Sequences Flanking Mutator (Mu) Transposons and Thereby Facilitate Gene Cloning -- Molecular Genetics and Epigenetics of CACTA Elements -- Activation Tagging Using the Maize En-I Transposon System for the Identification of Abiotic Stress Resistance Genes in Arabidopsis -- Reverse Genetics in Rice using Tos17 -- Identification and Applications of the Petunia Class II Act1/dTph1 Transposable Element System -- Transposon Display: A Versatile Method for Transposon Tagging -- Massive Indexed Parallel Identification of Transposon Flanking Sequences -- Use of Next Generation Sequencing (NGS) Technologies for the Genome-wide Detection of Transposition -- Overview of Repeat Annotation and de novo Repeat Identification -- Computational Methods for Identification of DNA Transposons -- TEnest 2.0: Computational Annotation and Visualization of Nested Transposable Elements.
520			_aTransposable elements have played a major role in shaping plant genome structure and gene expression.  Transposons not only drive sequence expansion, induce mutations and generate chromosome rearrangements, they also help to shape the epigenetic topology of the eukaryotic genome.  In Plant Transposable Elements: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study transposons. These methods include computational approaches to study the ancient transposon remnants that comprise the bulk of plant genomes, as well as laboratory techniques to identify recent and ongoing transposition events. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Plant Transposable Elements: Methods and Protocols seeks to aid scientists in the further study of transposons by providing essential background information and specific experimental protocols.
700	1		_aPeterson, Thomas _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627035699
776	0	8	_iPrinted edition: _z9781627035675
776	0	8	_iPrinted edition: _z9781493962716
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-568-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2013
999			_c234863 _d234863