000			03432nam a22003855i 4500
001			234874
003			ES-VaUE
005			20221220020630.0
007			cr nn 008mamaa
008			100301s1995 xxu| s |||| 0|eng d
020			_a9781592596317
024	7		_a10.1385/0896033112 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPatch-Clamp Applications and Protocols _cedited by Alan A. Boulton, Glen B. Baker, Wolfgang Walz.
250			_a1st edition 1995
264		1	_aTotowa, NJ _bHumana Press _c1995
300			_a1 recurso en línea (XVI, 316 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aNeuromethods _x1940-6045 _v26
505	0		_aTechnology of Patch- Clamp Electrodes -- Whole-Cell Patch-Clamp Recordings -- Pressure/Patch-Clamp Methods -- Cell-Free Ion-Channel Recording -- Perfusion of Patch Pipets -- Concentration Clamp Technique -- Perforated Patch-Clamp Technique -- The Loose Patch Voltage Clamp Technique -- Patch-Clamp Recording and RT-PCR on Single Cells -- Patch-Clamp Technique in Brain Slices -- Xenopus Oocyte Microinjection and Ion-Channel Expression.
520			_aE. Neher and B. Sakman were the first to monitor the opening and closing of single ion channels and membranes by conductance measurements. In 1976, they used firepolished micropipets with a tip diameter of 3-5 pm to record currents from a small patch of the membranbe of sk- etal muscles, thereby decreasing background membrane noise. In order to reduce the dominant source of background noise-the leakage shunt under the pipet rim between m- the muscle membrane had to be treated brane and gla- enzymatically. Despite these early limitations, a new te- nique was born -the patch-clamp technique. The final bre- through came in 1981 when the same authors, in collaboration with 0. P. Hamill, A. Marty, and F. J. Sigworth, developed the gigaohm seal. Not only did this improve the quality of recordings, it was now possible to gently pull the membrane patch with the attached pipet off the cell and study its trapped ion channels in isolation. Another offshoot of the gigaohm seal technique was the whole-cell patch-clamp technique, in which the patch is ruptured without breaking the seal. This technique is really a sophisticated voltage-clamp technique and also allows for the altering of cytoplasmic constituents if the experimenter so wishes. The first part of Patch-Clamp Applications and Protocols presents modern developments associated with the techn- ogy of patch-clamp electrodes, of cell-free ion channel reco- ing, and of the whole-cell patch-clamp technique.
700	1		_aBoulton, Alan A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aBaker, Glen B _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aWalz, Wolfgang _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489940643
776	0	8	_iPrinted edition: _z9780896033115
776	0	8	_iPrinted edition: _z9781489940636
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896033112 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1995
999			_c234874 _d234874