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008			100301s2002 xxu| s |||| 0|eng d
020			_a9781592591947
024	7		_a10.1385/1592591949 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aIn Vitro Mutagenesis Protocols _cedited by Jeff Braman.
250			_a2nd edition 2002
264		1	_aTotowa, NJ _bHumana Press _c2002
300			_a1 recurso en línea (XVI, 288 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v182
505	0		_aRapid and Reliable Site-Directed Mutagenesis Using Kunkel's Approach -- Site-Directed Mutagenesis Using Altered /gb-Lactamase Specificity -- Site-Directed Mutagenesis Facilitated by DpnI Selection on Hemimethylated DNA -- Multiple Site-Directed Mutagenesis In Vitro -- Two-Stage Polymerase Chain Reaction Protocol Allowing Introduction of Multiple Mutations, Deletions, and Insertions, Using QuikChangeTM Site-Directed Mutagenesis -- Efficient and Accurate Site-Directed Mutagenesis of Large Plasmids -- Combining Site-Specific Chemical Modification with Site-Directed Mutagenesis -- Site-Directed Mutagenesis Mediated by a Single Polymerase Chain Reaction Product -- Megaprimer Method for Polymerase Chain Reaction-Mediated Generation of Specific Mutations in DNA -- Generation of Epitope-Tagged Proteins by Inverse Polymerase Chain Reaction Mutagenesis -- Site-Directed Mutagenesis by Polymerase Chain Reaction -- Generation of Multiple Site-Specific Mutations by Polymerase Chain Reaction -- Phenotypic Expression of Polymerase Chain Reaction-Generated Random Mutations in a Foreign Gene After its Introduction into an Acinetobacter Chromosome by Natural Transformation -- Polymerase Chain Reaction-Mediated Mutagenesis in Sequences Resistant to Homogeneous Amplification -- Polymerase Chain Reaction-Based Signature-Tagged Mutagenesis -- High-Throughput Scanning Mutagenesis by Recombination Polymerase Chain Reaction -- In Vitro Scanning-Saturation Mutagenesis -- Random Transposon Mutagenesis of Large DNA Molecules in Escherichia coli -- Random Chromosomal Gene Disruption Using Cassette Mutagenesis -- Transplacement Mutagenesis -- Preparation of Transposon Insertion Lines and Determination of Insertion Sites in Arabidopsis Genome -- Evolutionary Molecular Engineering by Random Elongation Mutagenesis -- Random Mutagenesis for Protein Breeding -- DNA Shuffling and Family Shuffling for In Vitro Gene Evolution -- Mutagenic Polymerase Chain Reaction of Protein-Coding Genes for In Vitro Evolution.
520			_aIn the post-genome era, in vitro mutagenesis has emerged as the critically important tool used by molecular biologists in establishing the functions of components of the proteome. In this second edition of In Vitro Mutagenesis Protocols, active researchers with proven track records describe in stepwise fashion their advanced mutagenesis techniques. Each contributor focuses on improvements to conventional site-directed mutagenesis, with chapters being devoted to chemical site-directed mutagenesis; PCR-based mutagenesis and the modifications that allow high-throughput experiments; and mutagenesis based on gene disruption that is both in vitro- and in situ-based. Additional methods are provided for in vitro gene evolution; for gene disruption based on transposon, recombination, and cassette mutagenesis; and for facilitating the introduction of multiple mutations. Each readily reproducible technique includes detailed step-by-step instructions, tips on pitfalls to avoid, and notes on reagents and suppliers. Time-tested and highly practical, the techniques in In Vitro Mutagenesis Protocols, Second Edition offer today's molecular biologists a rich compendium of reliable and powerful techniques with which to illuminate the proteome.
700	1		_aBraman, Jeff _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617372483
776	0	8	_iPrinted edition: _z9780896039100
776	0	8	_iPrinted edition: _z9781489938947
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592591949 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2002
999			_c234881 _d234881