000			04839nam a22003735i 4500
001			234883
003			ES-VaUE
005			20221220020630.0
007			cr nn 008mamaa
008			130125s2013 xxu| s |||| 0|eng d
020			_a9781627032278
024	7		_a10.1007/978-1-62703-227-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMolecular Dermatology _bMethods and Protocols _cedited by Cristina Has, Cassian Sitaru.
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (XII, 460 páginas) _b84 ilustraciones, 59 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v961
505	0		_aMolecular Dermatology Comes of Age -- Molecular Diagnosis of Autoimmune Blistering Diseases -- Molecular Diagnosis of Genodermatoses -- Three-Dimensional Visualization of the Molecular Architecture of Cell-Cell Junctions In Situ by Cryo-Electron Tomography of Vitreous Sections -- 3D Visualization of Epidermal Langerhans Cells -- In Vivo Imaging of Lymph Node Lymphangiogenesis by Immuno-Positron Emission Tomography -- Optical Imaging of HPV Infection in a Murine Model -- Laser Scanning Microscopy Approach for Semiquantitation of In Vitro Dermal Particle Penetration -- Analysis of Cutaneous Somatic Mosaicism -- Global Proteome Analyses of SILAC Labeled Skin Cells -- MicroRNA Profiling During Human Keratinocyte Differentiation Using a Quantitative Real Time PCR Method -- Cell Death in the Skin: How to Study Its Quality and Quantity? -- In Vitro Pathogenicity Assay for Anti-Desmoglein Autoantibodies in Pemphigus -- Induction of Granulocyte-Dependent Dermal-Epidermal Separation by Autoantibodies Ex Vivo -- Analysis of Collective Invasion of Carcinoma Cells in a 3D Organotypic Model -- Isolation of Melanoma Tumor Initiating Cells from Surgical Tissues -- Detection, Enumeration, and Characterization of Immune Cells Infiltrating Melanoma Tumors -- Analysis of Cell Movement Between Skin and Other Anatomical Sites In Vivo Using Photoconvertible Fluorescent Protein "Kaede"-Transgenic Mice -- Reconstitution of Skin Fibrosis Development Using a Tissue Engineering Approach -- Bioengineered Skin Humanized Model of Psoriasis -- Induction of Contact Hypersensitivity in the Mouse Model -- Generation of Functional Multipotent Keratinocytes from Mouse Induced Pluripotent Stem Cells -- RNAi-Mediated Gene Function Analysis in Skin -- Mouse Models of Autoimmune Blistering Diseases Induced by the Passive Transfer of Antibodies -- Induction of Experimental Epidermolysis Bullosa Acquisita by Immunization with Murine Collagen VII -- Animal Model for Cutaneous Leishmaniasis -- Model for Generation of Large Numbers of Primary, Inflammatory Skin-Derived Neutrophils and Macrophages -- Zebrafish as a Model System to Study Heritable Skin Diseases -- Cell- and Protein-Based Therapy Approaches for Epidermolysis Bullosa -- RNA Trans-Splicing for Genodermatoses.
520			_aThe sustained skin research efforts over the past decades has led to the accumulation of a significant collection of information on skin structure and physiology as well as on the pathogenesis of cutaneous diseases.  In Molecular Dermatology: Methods and Protocols, leading experts in the field provide a collection of state-of-the-art reliable protocols covering a wide spectrum of techniques and experimental models, specific molecular assays and disease models, as well as overviews of diagnostic and research areas relevant to molecular dermatology.  As a volume in the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Comprehensive and authoritative, Molecular Dermatology: Methods and Protocols emphasizes the vital importance of skin research and collects the methodologies necessary to aid scientists in moving forward in this valuable field.
700	1		_aHas, Cristina _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aSitaru, Cassian _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627032261
776	0	8	_iPrinted edition: _z9781627032285
776	0	8	_iPrinted edition: _z9781493959778
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-227-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2013
999			_c234883 _d234883