000 04355nam a22003735i 4500
001 234885
003 ES-VaUE
005 20221220020630.0
007 cr nn 008mamaa
008 100301s2009 xxu| s |||| 0|eng d
020 _a9781603270090
024 7 _a10.1007/978-1-60327-009-0
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aHuman Embryogenesis
_bMethods and Protocols
_cedited by Julie Lafond, Cathy Vaillancourt.
250 _a1st edition 2009
264 1 _aTotowa, NJ
_bHumana Press
_c2009
300 _a1 recurso en línea (X, 312 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v550
505 0 _aIntroduction: What is Human Embryogenesis? -- Human Embryogenesis: Overview -- Studying Embryo Implantation: From Trophoblasts to Embryo -- Differential Gene Expression in the Uterus and Blastocyst During the Reactivation of Embryo Development in a Model of Delayed Implantation -- Coculture of Decidua and Trophoblast to Study Proliferation and Invasion -- Isolation and Culture of Term Human Cytotrophoblast Cells and In Vitro Methods for Studying Human Cytotrophoblast Cells' Calcium Uptake -- Endocrinology and Cell Signaling in Human Villous Trophoblast -- Gestation-Induced Uterine Vascular Remodeling -- Steriodogenesis and Reproductive System -- Placental and Fetal Steroidogenesis -- Current Methods in Investigating the Development of the Female Reproductive System -- A Revised and Improved Method for the Isolation of Seminiferous Tubule-Enriched Fractions that Preserves the Phosphorylated and Glycosylated Forms of Proteins -- A Novel Technical Approach for the Measurement of Individual ACAT-1 and ACAT-2 Enzymatic Activity in the Testis -- From Embryo to Fetal Development -- Genetic Dissection of Caenorhabditis elegans Embryogenesis Using RNA Interference and Flow Cytometry -- Immune System: Maturation of Myeloid Cells -- Functional Development of Human Fetal Gastrointestinal Tract -- Renal and Cardiac Na+-K+-ATPase and Aconitase in a Rat Model of Fetal Programming -- Embryotoxicity, in Vitro Fertilization, Cloning, and Ethics -- Assessment of Embryotoxicity Using Mouse Embryo Culture -- Fertilization In Vitro -- Animal Cloning by Somatic Cell Nuclear Transfer -- The Human Embryo: Ethical and Legal Aspects.
520 _aDespite its inherent controversy, the exploration of the human embryo can unlock many of the answers to our deepest biological questions. In Human Embryogenesis: Methods and Protocols, internationally recognized researchers contribute detailed methods to analyze various aspects of the embryogenesis process. While comprehensively covering subjects such as the molecular mechanisms of embryonic development, in vitro fertilization, cloning, and the laws and ethical considerations of working with embryos, the volume also addresses critical features of fetal and placental development as well as of uterine biology. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and state-of-the-art, Human Embryogenesis: Methods and Protocols provides a firm foundation for the successful analysis of the embryogenesis process and an easily accessible description of the limitations and advantages of the techniques proposed, certain to aid all those who wish to further unravel the mysteries of human embryogenesis.
700 1 _aLafond, Julie
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aVaillancourt, Cathy
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781607610762
776 0 8 _iPrinted edition:
_z9781603270083
776 0 8 _iPrinted edition:
_z9781627038324
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-009-0
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2009
999 _c234885
_d234885