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001			234889
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007			cr nn 008mamaa
008			150915s2015 xxu| s |||| 0|eng d
020			_a9781493929900
024	7		_a10.1007/978-1-4939-2990-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aWhole Genome Amplification _bMethods and Protocols _cedited by Thomas Kroneis.
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (X, 284 páginas) _b18 ilustraciones, 13 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1347
505	0		_aPrinciples of Whole Genome Amplification.- Bias in Whole Genome Amplification: Causes and Considerations.- The Single-Cell Lab or How to Perform Single-Cell Molecular Analysis -- Sample Preparation Methods Following Cell Search Approach Compatible of Single-Cell Whole Genome Amplification: An Overview -- Deterministic Whole Genome Amplification of Single Cells -- Construction of a DNA Library on Micro beads Using Whole Genome Amplification.- Heat-Induced Fragmentation and Adapter-Assisted Whole Genome Amplification Using Genome Plex® Single Cell Whole Genome Amplification Kit (WGA4).- Whole Genome Amplification by Isothermal Multiple Strand Displacement Using Phi29 DNA Polymerase.- Using Multiplex-PCR for Assessing the Quality of Whole Genome Amplified DNA.- Quality Control of Isothermal Amplified DNA Based on Short Tandem Repeat Analysis.- Laser-Micro dissection of FFPE Tissue Areas and Subsequent Whole Genome Amplification by Ampli1TM.- Whole Genome Amplification from Blood Spot Samples.- Analysis of Whole Mitogenomes from Ancient Samples.- Copy Number Variation Analysis by Array Analysis of Single Cells Following Whole Genome Amplification.- Whole Genome Amplification in Genomic Analysis of Single Circulating Tumor Cells.- Whole Genome Amplification of Labeled Viable Single Cells Suited for Array Comparative Genomic Hybridization.- Low-Volume On-Chip Single-Cell Whole Genome Amplification for Multiple Subsequent Analyses.- Detection and Characterization of Circulating Tumor Cells by the Cell Search Approach.
520			_aThis volume mirrors the holistic feature of whole genome amplification (WGA) technology by combining reviews, detailed basic methods, and advanced sample workflows. The first part of the book covers an overview of the development of WGA techniques throughout recent years including general considerations on bias in WGA, possible sample pre-enrichment strategies and how to run a single-cell lab. The second part focuses on major WGA methods and protocols that allow the assessment of WGA product quality. The final chapters contain advanced protocols and address issues such as sample preparation using laser-microdissection; WGA from partially degraded DNA (formalin-fixed paraffin embedded samples); circulating tumor cells; and ancient samples. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Whole Genome Amplification: Methods and Protocols will serve as a rich source of detailed information and inspiration, and will help researchers, both new and experienced, yield successful results. .
700	1		_aKroneis, Thomas _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493929894
776	0	8	_iPrinted edition: _z9781493929917
776	0	8	_iPrinted edition: _z9781493948468
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2990-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c234889 _d234889