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008			151030s2016 xxu| s |||| 0|eng d
020			_a9781493931453
024	7		_a10.1007/978-1-4939-3145-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aYeast Cytokinesis _bMethods and Protocols _cedited by Alberto Sanchez-Diaz, Pilar Perez.
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XI, 395 páginas) _b83 ilustraciones, 52 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1369
505	0		_aTime-lapse Fluorescence Microscopy Of Budding Yeast Cells -- Real-time Visualization and Quantification of Contractile Ring Proteins in Single Living Cells -- Fluorescence Recovery After Photo-bleaching (FRAP) and Fluorescence Loss in Photo-bleaching (FLIP) Experiments to Study Protein Dynamics during Budding Yeast Cell Division -- High-Speed Super Resolution Imaging of Live Fission Yeast Cells -- Monitoring Chitin Deposition during Septum Assembly in Budding Yeast -- Imaging Septum Formation by Fluorescence Microscopy -- Visualization of Cytokinesis Events in Budding Yeast by Transmission Electron Microscopy -- Visualization of Fission Yeast Cells By Transmission Electron Microscopy -- Characterization of Septin Ultrastructure in Budding Yeast Using Electron Tomography -- Isolation of Cytokinetic Actomyosin Rings from Saccharomyces cerevisiae and Schizosaccharomyces pombe -- Measurements of Myosin-II motor Activity during Cytokinesis In Fission Yeast -- In Vitro Biochemical Characterization of Cytokinesis Actin Binding Proteins -- Characterization of Cytokinetic F-BARs and other Membrane Binding Proteins -- Analysis of Three-Dimensional Structures of Exocyst Components -- Analysis of Rho-GTPase Activity during Budding-yeast Cytokinesis -- Detection of Phosphorylation Status of Cytokinetic Components -- Studying Protein-Protein Interactions in Budding Yeast Using Co-Immunoprecipitation -- Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening Of Suppressor Genes -- Synchronization of the Budding Yeast Saccharomyces cerevisiae -- Fission Yeast Cell Cycle Synchronization Methods -- A Review of Fluorescent Proteins for Use in Yeast -- Visualisation and Image Analysis of Yeast Cells -- Toolbox for Protein Structure Prediction -- From Structure to Function. A Comprehensive Compendium of Tools to Unveil Protein Domains and Understand their Role in Cytokinesis.
520			_aThis volume presents protocols on yeast cytokinesis that will help Molecular and Cellular Biology researchers in the use of these microorganisms to approach the study of general or specific key questions in cytokinesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Yeast Cytokinesis: Methods and Protocols provides practical and step-by-step detailed protocols useful for a wide audience ranging from experienced researchers to beginners in the use of yeasts.
700	1		_aSanchez-Diaz, Alberto _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aPerez, Pilar _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493931446
776	0	8	_iPrinted edition: _z9781493931460
776	0	8	_iPrinted edition: _z9781493979899
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3145-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c234894 _d234894