000			03676nam a22003735i 4500
001			234897
003			ES-VaUE
005			20221220020631.0
007			cr nn 008mamaa
008			100301s2002 xxu| s |||| 0|eng d
020			_a9781592592739
024	7		_a10.1385/1592592732 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPCR Mutation Detection Protocols _cedited by Bimal D. M. Theophilus, Ralph Rapley.
250			_a1st edition 2002
264		1	_aTotowa, NJ _bHumana Press _c2002
300			_a1 recurso en línea (X, 214 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v187
505	0		_aAgarose and Polyacrylamide Gel Electrophoresis -- Internal Labeling of DNA Probes -- End-Labeling of DNA Probes -- Southern Blotting of Agarose Gels by Capillary Transfer -- Restriction Fragment Length Polymorphism -- PCR -- Allele-Specific Oligonucleotide PCR -- Long-Range PCR -- Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing -- Cycle Sequencing of PCR Products -- Fluorescent In Situ Hybridization -- The Protein Truncation Test -- Mutation Detection in Factor VIII cDNA from Lymphocytes of Hemophilia A Patients by Solid Phase Fluorescent Chemical Cleavage of Mismatch -- Denaturing Gradient Gel Electrophoresis -- Conformation-Sensitive Gel Electrophoresis -- SSCP/Heteroduplex Analysis -- Cleavase® Fragment Length Polymorphism Analysis for Genotyping and Mutation Detection -- Automated Genotyping Using the DNA MassArray™ Technology -- An Introduction to Bioinformatics.
520			_aApplication of DNA technology to the identification of disease-causing mutations has become widespread in recent years. PCR Mutation Detection Protocols, provides biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Additional coverage is given to the direct use of DNA sequencing as a detection method in its own right and to the characterization of mutations previously located by other screening techniques. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution. Comprehensive and highly practical, PCR Mutation Detection Protocols assures both seasoned and novice investigators access to the highly productive and readily reproducible PCR-based mutation detection methods, techniques that are laying the groundwork for many of today's major scientific and medical advances.
700	1		_aTheophilus, Bimal D. M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aRapley, Ralph _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617371004
776	0	8	_iPrinted edition: _z9780896036178
776	0	8	_iPrinted edition: _z9781489938817
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592592732 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2002
999			_c234897 _d234897