000			04233nam a22003735i 4500
001			234908
003			ES-VaUE
005			20221220020632.0
007			cr nn 008mamaa
008			161028s2017 xxu| s |||| 0|eng d
020			_a9781493965458
024	7		_a10.1007/978-1-4939-6545-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aCohesin and Condensin _bMethods and Protocols _cedited by Kyoko Yokomori, Katsuhiko Shirahige.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XII, 276 páginas) _b41 ilustraciones, 33 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1515
505	0		_aA Sister Chromatid Cohesion Assay Using Xenopus Egg Extracts -- An In Vitro Assay for Monitoring Topological DNA Entrapment by the Chromosomal Cohesin Complex -- Biochemical and Functional Assays of Human Cohesin-Releasing Factor Wapl -- Detection of Cohesin SUMOylation In Vivo -- Analysis of Meiotic Sister Chromatid Cohesion in Caenorhabditis elegans -- Protein and Chromosome Analysis in Mammalian Meiocytes -- Resolving the Genomic Localization of the Kollerin Cohesin-Loader Complex -- Measuring Sister Chromatid Cohesion Protein Genome Occupancy in Drosophila melanogaster by ChIP-seq -- A Dual-Color Reporter Assay of Cohesin-Mediated Gene Regulation in Budding Yeast Meiosis -- Methods to Study the Atypical Roles of DNA Repair and SMC Proteins in Gene Silencing -- Zebrafish as a Model to Study Cohesin and Cohesinopathies -- Analysis of Cohesin Function in Gene Regulation and Chromatin Organization in Interphase -- Using Fluorescent Reporters in Conjunction with Cytometry and Statistics to Assess Nuclear Accumulation of Ribosomal Proteins -- The Use of Laser Microirradiation to Investigate the Roles of Cohesins in DNA Repair -- A Protocol for Measuring Mitotic Chromosome Condensation Quantitatively in Fission Yeast Cells -- ChIP-seq Analysis of Condensin Complex in Cultured Mammalian Cells.
520			_aThe volume provides comprehensive, state-of-the-art experimental techniques that are now available to dissect the molecular mechanisms of regulation and function of cohesin and the related factor condensin in vitro and in vivo across different model organisms, as well as in human cells. Cohesin and Condensin: Methods and Protocols is divided into three parts: Part I explores various in vitro and in vivo systems used to study the fundamental mechanism of cohesin regulation in mitosis and meiosis; Part II summarizes experimental systems in a variety of organisms that are used to address interphase functions of cohesin and Nipbl in gene regulation and chromatin interaction, ribosome biogenesis and DNA repair, which contribute significantly to cohesion-associated disorders; Part III covers related condensin complex and describes techniques to study its role in mitosis and interphase. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Cohesin and Condensin: Methods and Protocols is a valuable resource for diverse audiences with interests in the relationship between chromatin organization and genomic functions.
700	1		_aYokomori, Kyoko _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aShirahige, Katsuhiko _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493965434
776	0	8	_iPrinted edition: _z9781493965441
776	0	8	_iPrinted edition: _z9781493982325
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6545-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c234908 _d234908