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001			234912
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008			161124s2017 xxu| s |||| 0|eng d
020			_a9781493965366
024	7		_a10.1007/978-1-4939-6536-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aCampylobacter jejuni _bMethods and Protocols _cedited by James Butcher, Alain Stintzi.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 274 páginas) _b45 ilustraciones, 27 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1512
505	0		_aCampylobacter jejuni Isolation/Enumeration from Environmental Samples -- Recovery of Campylobacter spp. from Food and Environmental Sources -- Methods for Isolation, Purification, and Propagation of Bacteriophages of Campylobacter jejuni -- Drug Resistance Detection in Campylobacter -- Method of Peptide Nucleic Acid (PNA)-Mediated Antisense Inhibition of Gene Expression in Campylobacter jejuni -- Identification of Ligand-receptor Interactions: Ligand Molecular Arrays, SPR and NMR Methodologies -- Characterization of High Affinity Iron Acquisition Systems in Campylobacter jejuni -- Method for the Successful Crystallization of the Ferric Uptake Regulator from Campylobacter jejuni -- Methods for Initial Characterization of Campylobacter jejuni Bacteriophages -- Methods to Assess the Direct Interaction of C. jejuni with Mucins -- Methods to Study Campylobacter jejuni Adherence to and Invasion of Host Epithelial Cells -- Assays to Study the Interaction of Campylobacter jejuni with the Mucosal Surface -- Characterisation of Ligand-receptor Interactions: Chemotaxis, Biofilm, Cell Culture Assays, and Animal Model Methodologies -- Using Galleria Mellonella as an Infection Model for Campylobacter jejuni Pathogenesis -- Mouse Models for Campylobacter jejuni Colonization and Infection -- Metabolomic Analysis of Campylobacter jejuni by Direct Injection Electrospray Ionisation Mass Spectrometry -- Methods for Genome-wide Methylome Profiling of Campylobacter jejuni -- Characterizing Glycoproteins by Mass Spectrometry in Campylobacter jejuni -- Methods for Proteome Analysis of Campylobacter jejuni using 2-D Electrophoresis -- Analyzing Prokaryotic RNA-seq Data: A Case Study Identifying Holo-Fur Regulated Genes in Campylobacter jejuni -- Generation and Screening of an Insertion Sequencing-compatible Mutant Library of Campylobacter jejuni. .
520			_aCampylobacter jejuni (C. jejuni) is often regarded as the one of the most common causes of bacterial gastroenteritis worldwide. The goal of this volume is to highlight key protocols for working with C. jejuni. In particular, chapters aim to highlight recent developments with regards to in vivo models for C. jejuni pathogenesis, different approaches to isolate Campylobacter, and a systems biology approach for studying the effect of all potential Campylobacter gene mutants.Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, <Campylobacter jejuni: Methods and Protocols encourages existing Campylobacter researchers to employ novel methods to further their own research and also encourages new researchers to include Campylobacter in their future research initiatives.
700	1		_aButcher, James _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aStintzi, Alain _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493965342
776	0	8	_iPrinted edition: _z9781493965359
776	0	8	_iPrinted edition: _z9781493982295
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6536-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c234912 _d234912