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020 _a9781617795138
024 7 _a10.1007/978-1-61779-513-8
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aMucins
_bMethods and Protocols
_cedited by Michael A. McGuckin, David J. Thornton.
250 _a1st edition 2012
264 1 _aTotowa, NJ
_bHumana Press
_c2012
300 _a1 recurso en línea (XI, 329 páginas)
_b48 ilustraciones, 4 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v842
505 0 _aMucin Methods: Genes Encoding Mucins And Their Genetic Variation With A Focus On Gel Forming Mucins -- Gel-forming and Cell-associated Mucins - preparation for Structural and Functional Studies -- Detecting, Visualising and Quantifying Mucins -- Mass Spectrometric Analysis of Mucin Core Proteins -- O-Glycoprotein Biosynthesis:  Site Localization by Edman Degradation and Site Prediction Based on Random Peptide Substrates -- Analysis of Assembly of Secreted Mucins -- MUC1 Membrane Trafficking: Protocols for Assessing Biosynthetic Delivery, Endocytosis, Recycling and Release Through Exosomes -- Glycomic Work-flow for Analysis of Mucin O-linked Oligosaccharides -- Glycomic Work-flow for Analysis of Mucin O-linked Oligosaccharides -- O-Glycomics: Profiling and Structural Analysis of Mucin-type O-linked Glycans -- O-Glycoproteomics: Site-specific O-glycoprotein Analysis by CID/ETD Electrospray Ionization Tandem Mass Spectrometry and Top-Down Glycoprotein Sequencing by In-Source Decay MALDI Mass Spectrometry -- Analysing Mucin Degradation -- Assessment of Mucus Thickness and Production In Situ -- Preservation of Mucus in Histological Sections, Immunostaining of Mucins in Fixed Tissue and Localization of Bacteria with FISH -- In vitro Measurements of Mucus Secretion by Colon Explants -- Establishment of Respiratory Air-Liquid Interface Cultures and their Use in Studying Mucin Production, Secretion, and Function -- Studying Mucin Secretion from Human Bronchial Epithelial Cell Primary Cultures -- Assessment of Intracellular Mucin Content In Vivo -- Techniques for Assessment of Interactions of Mucins with Microbes and Parasites In Vitro and In Vivo -- Assessing Mucin Expression and Function in Human Ocular Surface Epithelia In Vivo and In Vitro.
520 _aEpithelial mucins are large complex cell surface and secreted glycoproteins produced by mucosal epithelial cells.  In, Mucins: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study Mucins. These include methods and techniques  for the best approaches to analysing each specific area of mucin biochemistry, physiology and biophysics before providing individual detailed experimental protocols together with troubleshooting and interpretation tips. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Mucins: Methods and Protocols is designed to be a useful resource for those entering the mucin field and to facilitate those already studying mucins to broaden their experimental approaches to understanding mucosal biology.
700 1 _aMcGuckin, Michael A
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aThornton, David J
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617795121
776 0 8 _iPrinted edition:
_z9781617795145
776 0 8 _iPrinted edition:
_z9781493958863
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-513-8
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2012
999 _c234932
_d234932