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001			234933
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007			cr nn 008mamaa
008			170714s2017 xxu| s |||| 0|eng d
020			_a9781493971879
024	7		_a10.1007/978-1-4939-7187-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aFast Detection of DNA Damage _bMethods and Protocols _cedited by Vladimir V. Didenko.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (X, 216 páginas) _b42 ilustraciones, 24 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1644
505	0		_aExpress FRET Labeling and Analysis of Phagocytic Clearance -- Rapid Assessment of Genotoxicity by Flow Cytometric Detection of Cell Cycle Alterations -- Ultrasound Imaging of DNA-Damage Effects in Live Cultured Cells and in Brain Tissue -- Ultrasound Imaging of Apoptosis: Spectroscopic Detection of DNA-Damage Effects In Vivo -- Fluorochrome-Labeled Inhibitors of Caspases: Expedient In Vitro and In Vivo Markers of Apoptotic Cells for Rapid Cytometric Analysis -- The Fast-halo Assay for the Detection of DNA Aamage -- Rapid Detection of Bacterial Susceptibility or Resistance to Quinolones -- Rapid Detection of Apoptosis in Cultured Mammalian Cells -- Quick Detection of DNase II-type Breaks in Formalin-Fixed Tissue Sections -- Express γ-H2AX Immunocytochemical Detection of DNA damage -- Rapid detection of γ-H2AX by Flow Cytometry in Cultured Mammalian Cells Firsanov -- Rapid Detection of DNA Strand Breaks in Apoptotic Cells by Flow- and Image- Cytometry -- Fast Micromethod: Determination of DNA Integrity in Cell Suspensions and in Solid Tissues -- Quantification of DNA Damage and Repair in Mitochondrial, Nuclear, and Bacterial Genomes by Real-Time PCR -- Zebra Tail Amplification: Accelerated Detection of Apoptotic Blunt Ended DNA Breaks by In Situ Ligation -- Twelve Gel Comet Assay Format for Quick Examination of DNA Damage and Repair -- Immunofluorescence Analysis of γ-H2AX Foci in Mammalian Fibroblasts at Different Phases of the Cell Cycle -- RAPD-PCR as a Rapid Approach for the Evaluation of Genotoxin-Induced Damage to Bacterial DNA -- Rapid Detection of γ-H2Av Foci in ex vivo MMS-treated Drosophila Imaginal Discs. .
520			_aThis volume presents a comprehensive collection of quick assays for the detection of nuclear and mitochondrial DNA damage and its effects in live and fixed cells and tissues, and in bacterial genomes. Although, such rapid techniques are in demand in the "research trenches" they are not covered well in the literature. This volume is the first such compendium of the time-saving techniques for detection of DNA damage and its direct physiological outcomes including apoptosis, necrosis and phagocytic clearance. The volume demonstrates all levels of detection, starting from the molecular level up to the level of the entire live organism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Fast Detection of DNA Damage: Methods and Protocols aims to provide easily reproducible techniques requiring only few steps to perform.
700	1		_aDidenko, Vladimir V _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493971855
776	0	8	_iPrinted edition: _z9781493971862
776	0	8	_iPrinted edition: _z9781493984114
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7187-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c234933 _d234933