000			03250nam a22003855i 4500
001			234936
003			ES-VaUE
005			20221220020633.0
007			cr nn 008mamaa
008			100301s2002 xxu| s |||| 0|eng d
020			_a9781592592760
024	7		_a10.1385/1592592767 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPatch-Clamp Analysis _bAdvanced Techniques _cedited by Wolfgang Walz, Alan A. Boulton, Glen B. Baker.
250			_a1st edition 2002
264		1	_aTotowa, NJ _bHumana Press _c2002
300			_a1 recurso en línea (XII, 345 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aNeuromethods _x1940-6045 _v35
505	0		_aTechnology of Patch-Clamp Electrodes -- Whole-Cell Patch-Clamp Recordings -- Single-Channel Recording -- Combined Fluorometric and Electrophysiological Recordings -- Patch-Clamp Techniques Applied To Brain Slices -- Perforated Patch-Clamp Technique -- Fast-Drug Application -- Pipet Perfusion Methods -- Patch-Cram Detection of Cyclic GMP in Intact Cells -- Loose-Patch Voltage-Clamp Technique -- Recording from Macropatches -- Combining Patch-Clamp Techniques with RT-PCR.
520			_aThe use of the patch pipet has moved from a specialized to a mainstream method that can be combined with other techniques to perform complex and novel operations. In Patch-Clamp Analysis: Advanced Techniques, Wolfgang Walz and his colleagues bring together a team of leading academic researchers to describe in reproducible detail all the most important patch-clamp methods. Beginning with updated basic patch-clamp techniques, these hands-on experts present the newest developments, ranging from fast external solution switching and the switching of the pipet solution during recording, to the loose-patch, the perforated-patch, and the patch-cram detection technique. Coverage of these advanced techniques combines their molecular biology and imaging to produce the patch pipet with RT-PCR and fluorometric techniques. All the methods included describe the theoretical background, offer complete startup help, and provide tips on solving common problems and analyzing data. Comprehensive and highly practical, Patch-Clamp Analysis: Advanced Techniques surveys all the major applications that involve patch pipets and sets the stage for the emergence of today's new applications involving genomics, proteomics, and sophisticated imaging techniques.
700	1		_aWalz, Wolfgang _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aBoulton, Alan A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aBaker, Glen B _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489938565
776	0	8	_iPrinted edition: _z9781588290137
776	0	8	_iPrinted edition: _z9781489938558
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592592767 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2002
999			_c234936 _d234936