000			04232nam a22003495i 4500
001			234944
003			ES-VaUE
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007			cr nn 008mamaa
008			100301s2006 xxu| s |||| 0|eng d
020			_a9781597450683
024	7		_a10.1385/1597450685 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPRINS and In Situ PCR Protocols _cedited by Franck Pellestor.
250			_a2nd edition 2006
264		1	_aTotowa, NJ _bHumana Press _c2006
300			_a1 recurso en línea (XVI, 244 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v334
505	0		_aBasic Methodology -- New Rapid Multicolor PRINS Protocol -- PRINS Combined With Peptide Nucleic Acid Labeling -- PRINS Combined With Indirect Immunofluorescence -- PRINS for the Detection of Unique Sequences -- Single-Copy and Point Mutation Analysis (Rolling-Circle PRINS) -- Analysis of Sperm Aneuploidy by PRINS -- In Situ PCR Protocols -- Research and Clinical Applications -- Detection and Sizing of Telomeric and Other Simple Repeats by Dideoxy-PRINS -- PRINS Evaluation of Chromosome Instability in Mammalian Cells by Detection of Repetitive DNA Sequences in Micronuclei -- PRINS for the Detection of Gene Deletions in Cancer -- Combined Fluorescence In Situ Hybridization and PRINS for the Analysis of the Dystrophin Gene -- Brain Tissue Preparations for Chromosomal PRINS Labeling -- PRINS on Plant Chromosomes -- Dual-Color PRINS for In Situ Detection of Fetal Cells in Maternal Blood -- PRINS as an Efficient Tool for Aneuploidy Assessment in Human Oocytes and Preimplantation Embryos -- Microdissection With PCR In Situ -- Application of Reverse Transcription In Situ PCR in Cancer Analysis -- In Situ Reverse Transcription PCR on Plant Tissues -- In Situ PCR for the Detection of Human Cytomegalovirus in Suspension Cells During the Latent Phase of Infection -- Development and Adaptation of the PRINS Technology -- Latest Developments in In Situ PCR.
520			_aCarrying on the high standards of the much-praised first edition, leading researchers and clinical investigators have completely revised and updated PRINS and In Situ PCR Protocols to take advantage of the many new developments and applications that have occurred for cellular and molecular investigations. The authors contribute proven protocols for many variations of PRINS and in situ PCR, including a new, fast multicolor PRINS method for identifying human chromosomes, PRINS protocols for in situ detection of unique sequences and point mutations, a new dideoxy PRINS procedure, and in situ PCR and PRINS protocols for plants. They describe, in detail, various applications of PRINS in human cells (detection of gene deletions in cancer, detection of fetal cells in maternal blood, and assessment of aneuploidy in brain tissues and embryos) as well as in plant cells. Readily reproducible in situ PCR techniques are also presented for the detection of cytomegalovirus and for use in combination with microdissection. In situ RT-PCR techniques are also given for use in plant or cancer investigation. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, PRINS and In Situ PCR Protocols offers molecular biologists, cytogeneticists, and cytologists a cutting-edge guide to today's new in situ labeling technology.
700	1		_aPellestor, Franck _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617376368
776	0	8	_iPrinted edition: _z9781588295491
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1597450685 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2006
999			_c234944 _d234944