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020 _a9781592596478
024 7 _a10.1385/1592596479
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aAtomic Force Microscopy
_bBiomedical Methods and Applications
_cedited by Pier Carlo Braga, Davide Ricci.
250 _a1st edition 2004
264 1 _aTotowa, NJ
_bHumana Press
_c2004
300 _a1 recurso en línea (XIV, 394 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v242
505 0 _aThe Basics of Atomic Force Microscopy -- How the Atomic Force Microscope Works -- Imaging Methods in Atomic Force Microscopy -- Recognizing and Avoiding Artifacts in AFM Imaging -- Advanced Biosensing Using Micromechanical Cantilever Arrays -- Morphostructural Analysis of Cellular Structures -- Analysis of Human Fibroblasts by Atomic Force Microscopy -- Corneal Tissue Observed by Atomic Force Microscopy -- AFM Study of Surface Structure Changes in Mouse Spermatozoa Associated With Maturation -- Calculation of Cuticle Step Heights from AFM Images of Outer Surfaces of Human Hair -- Imaging Living Chondrocyte Surface Structures With AFM Contact Mode -- Growth Cones of Living Neurons Probed by Atomic Force Microscopy -- Evaluating Demineralization and Mechanical Properties of Human Dentin With AFM -- Applying Atomic Force Microscopy to Studies in Cardiac Physiology -- Imaging Bacterial Shape, Surface, and Appendages Before and After Treatments With Antibiotics -- Subcellular Structures Investigation -- Visualizing Nuclear Structure In Situ by Atomic Force Microscopy -- Imaging Surface and Submembranous Structures in Living Cells With the Atomic Force Microscope -- Atomic Force Microscopy of Protein Complexes -- Atomic Force Microscopy of Interfacial Monomolecular Films of Pulmonary Surfactant -- High-Resolution Analysis of the 3D Organization of Human Metaphase Chromosomes -- Shape and Volume of Living Aldosterone-Sensitive Cells Imaged with the Atomic Force Microscope -- Localization of Epithelial Sodium Channels by Atomic Force Microscopy -- High-Resolution Imaging of Bacteriorhodopsin by Atomic Force Microscopy -- Functional Investigations with AFM -- Measurement of Mechanical Properties of Intact Endothelial Cells in Fresh Arteries -- Observation of Oxidative Stress on Yeast Cells -- Lymphoblastoid Cells Exposed to Low-Frequency Magnetic Fields -- Sample Preparation Method for Observing RNA Polymerase Activity by Atomic Force Microscopy -- Atomic Force Microscopy of ?-Amyloid -- How to Build Up Biosensors With the Cantilever of the Atomic Force Microscope -- Measurement of Single Molecular Interactions by Dynamic Force Microscopy.
520 _aAlthough atomic force microscopy (AFM) offers many significant advantages over the conventional microscopies used in the biological and medical sciences, its use is more familiar to physicists and engineers than to biomedical researchers. In Atomic Force Microscopy: Biomedical Methods and Applications, highly experienced physicians and biologists clearly explain the basic technical knowledge needed to use AFM and demonstrate its multifarious uses in biomedicine and the life sciences. The applications range widely from morphostructural analyses of cellular structures, to the investigation of subcellular structures, to functional investigations, and reveal a powerful new way of looking at biological samples. Each tested protocol includes step-by-step instructions to ensure successful experimental results, background material on the principle behind the technique, tips on troubleshooting and avoiding known pitfalls, and notes on how to distinguish artifacts from useful data. The methods clearly demonstrate the advantages of AFM over traditional life science microscopy, among them simultaneous very high magnification and resolution, minimal tissue and cell preparation, and the ability to obtain different views of the sample from a single data collection. Cutting-edge and highly practical, Atomic Force Microscopy: Biomedical Methods and Applications will help all investigators in biology and medicine open a new microscopic world, develop novel applications, and apply this powerful technology productively in their own work.
700 1 _aBraga, Pier Carlo
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aRicci, Davide
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781489939319
776 0 8 _iPrinted edition:
_z9781588290946
776 0 8 _iPrinted edition:
_z9781489939302
776 0 8 _iPrinted edition:
_z9781617790973
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592596479
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2004
999 _c234946
_d234946