000			03382nam a22003615i 4500
001			234950
003			ES-VaUE
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007			cr nn 008mamaa
008			140911s2014 xxu| s |||| 0|eng d
020			_a9781493914869
024	7		_a10.1007/978-1-4939-1486-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aProtein Design _bMethods and Applications _cedited by Valentin Köhler.
250			_a2nd edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (X, 279 páginas) _b76 ilustraciones, 55 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1216
505	0		_aDe novo Design of Stable α-helices -- Design of Monomeric Water-soluble b-hairpin and β-sheet Peptides -- Combination of Theoretical and Experimental Approaches for the Design and Study of Fibril-forming Peptides -- Posttranslational Incorporation of Non-canonical Amino Acids in the RNase S System by Semisynthetic Protein Assembly -- Design, Synthesis, and Study of Fluorinated Proteins -- High-Quality Combinatorial Protein Libraries Using the Binary Patterning Approach -- Methods for Library-Scale Computational Protein Design -- Symmetric Protein Architecture in Protein Design: Top-Down Symmetric Deconstruction -- Identification of Protein Scaffolds for Enzyme Design using Scaffold Selection -- Computational Design of Novel Enzymes without Cofactors -- De novo Design of Peptide Scaffolds as Novel Pre-organised Ligands for Metal-ion Coordination -- Computational Design of Metalloproteins -- Incorporation of Modified and Artificial Cofactors into Naturally Occurring Protein Scaffolds -- Computational Redesign of Metalloenzymes for Catalyzing New Reactions.
520			_a Protein Design: Method and Applications, Second Edition expands upon the previous edition with current, detailed ideas on how to approach a potential protein design project. With new chapters on metals as structure-forming elements and functional sites, the design and characterization of fluorinated proteins, top-down symmetric deconstruction and the design of protein libraries and novel or repurposed enzymes. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Thorough and intuitive, Protein Design: Method and Applications, Second Edition provides a number of practical protocols and instructive reviews to aid in the creation of new experiments.
700	1		_aKöhler, Valentin _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493914876
776	0	8	_iPrinted edition: _z9781493914852
776	0	8	_iPrinted edition: _z9781493944088
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1486-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2014
999			_c234950 _d234950