000			03283nam a22003735i 4500
001			234951
003			ES-VaUE
005			20221220020634.0
007			cr nn 008mamaa
008			100301s1993 xxu| s |||| 0|eng d
020			_a9781603274074
024	7		_a10.1007/978-1-60327-407-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
100	1		_aPassonneau, Janet V. _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut
245	1	0	_aEnzymatic Analysis _bA Practical Guide _cby Janet V. Passonneau, Oliver H. Lowry.
250			_a1st edition 1993
264		1	_aTotowa, NJ _bHumana Press _c1993
300			_a1 recurso en línea (X, 403 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aBiological Methods
505	0		_aGeneral Principles -- Pyridine Nucleotides -- A Kinetics Primer for the Tissue Analyst -- Constriction Pipets -- Preparation of Tissues for Analysis -- Specific Methods and Procedures -- Enzymatic Cycling -- A Collection of Metabolite Assays -- A Collection of Enzyme Assays -- Improvement, Modification, Adaptation, Troubleshooting, and Development of New Methods -- Quantitative Histochemistry -- Preparation of Tissue and Sections -- Dissection and Histological Control -- The Quartz Fiber Fishpole Balance -- Histochemical Analyses.
520			_aEnzymatic Analysis: A Practical Guide is a multipurpose manual of laboratory methods. It offers a systematic scheme for the analysis of biological materials from the level of the whole organ down to the single cell and beyond. It is intended as a guide to the development of new methods, to the refinement of old ones, and to the adaptation in general of methods to almost any scale of sensitivity. As some may realize, the book is a sequel to A Flexible System of Enzymatic Analysis, originally published in 1972. The major changes, other than an appropriate interchange of authors, consist of a wholly new chapter of methods and protocols for measuring enzymes, the addition of 13 new entries in the metabolite chapter, and a much superior chapter on enzymatic cycling. With considerable nostalgia, we have switched from DPN and TPN to NAD and NADP nomenclature, which no doubt will make Otto Warburg turn over in his grave. The incentives for the methodology in this book came from the rigorous demands of quantitative histochemistry and cytochemistry. These demands are specificity, simplicity, flexibility, and, of course, sensitivity-all likewise desirable attributes of methods for other purposes. The specificity is provided by the use of enzyme methods. Simplicity is achieved by leading all reactions to a final pyridine nucleotide step.
700	1		_aLowry, Oliver H. _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut
776	0	8	_iPrinted edition: _z9781489940148
776	0	8	_iPrinted edition: _z9780896032385
776	0	8	_iPrinted edition: _z9781489940131
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-407-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1993
999			_c234951 _d234951