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020 _a9781592596492
024 7 _a10.1385/1592596495
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aGene Delivery to Mammalian Cells
_bVolume 1: Nonviral Gene Transfer Techniques
_cedited by William C. Heiser.
250 _a1st edition 2004
264 1 _aTotowa, NJ
_bHumana Press
_c2004
300 _a1 recurso en línea (XV, 301 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v245
505 0 _aDelivery Using Chemical Methods -- Chemical Methods for DNA Delivery -- Gene Transfer into Mammalian Cells Using Calcium Phosphate and DEAE-Dextran -- DNA Delivery to Cells in Culture Using Peptides -- DNA Delivery to Cells in Culture Using PNA Clamps -- Dendrimer-Mediated Cell Transfection in Vitro -- DNA Delivery to Cells in Culture Using Cationic Liposomes -- Formulation of Synthetic Gene Delivery Vectors for Transduction of the Airway Epithelium -- Cationic Liposome-Mediated DNA Delivery to the Lung Endothelium -- Delivery of DNA to Tumor Cells Using Cationic Liposomes -- Delivery of Transposon DNA to Lungs of Mice Using Polyethyleneimine-DNA Complexes -- Delivery Using Physical Methods -- Gene Delivery Using Physical Methods -- Gene Delivery to Mammalian Cells by Microinjection -- Delivery of DNA to Cells in Culture Using Particle Bombardment -- Delivery of DNA to Skin by Particle Bombardment -- Biolistic Transfection of Cultured Organotypic Brain Slices -- Efficient Electroporation of Mammalian Cells in Culture -- Delivery of DNA to Skin by Electroporation -- In Vivo DNA Electransfer in Skeletal Muscle -- Electrically Mediated Plasmid DNA Delivery to Solid Tumors In Vivo -- Hydrodynamic Delivery of DNA -- Naked DNA Gene Transfer in Mammalian Cells -- Microparticle Delivery of Plasmid DNA to Mammalian Cells -- DNA Delivery to Cells in Culture Using Ultrasound -- DNA Delivery to Cells In Vivo by Ultrasound.
520 _aWhether to assess the function of new genes identified from the Human Genome Project or to apply gene therapy successfully, it is often necessary to deliver genes to specific cells. In Gene Delivery to Mammalian Cells, highly experienced researchers describe in great detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1: Nonviral Gene Transfer Techniques focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, and hydrodynamics. Each protocol contains step-by-step instructions, along with background notes, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. Introductory chapters review the delivery methods presented, discussing their advantages and disadvantages, how they have been used successfully for gene delivery, and the future of their technology. An accompanying volume, Volume 2: Viral Gene Transfer Techniques, details procedures for delivering genes to cells in vitro and in vivo. Comprehensive and cutting-edge, Gene Delivery to Mammalian Cells, volumes 1 and 2, offers biomedical specialists in industry and academia an unrivaled collection of reliable methods for the chemical, physical, and viral delivery of genes to mammalian cells, all prepared to save laboratory time and ensure experimental success.
700 1 _aHeiser, William C
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617373497
776 0 8 _iPrinted edition:
_z9781588290861
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592596495
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2004
999 _c234953
_d234953