| 000 | 05412nam a22003735i 4500 | ||
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| 001 | 234954 | ||
| 003 | ES-VaUE | ||
| 005 | 20221220020634.0 | ||
| 007 | cr nn 008mamaa | ||
| 008 | 100304s2010 gw | s |||| 0|eng d | ||
| 020 | _a9783642011443 | ||
| 024 | 7 |
_a10.1007/978-3-642-01144-3 _2doi |
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| 040 |
_aES-MaUEC _bspa _cES-MaUEC |
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| 245 | 1 | 0 |
_aAntibody Engineering Volume 1 _cedited by Roland E. Kontermann, Stefan Dübel. |
| 250 | _a2nd edition 2010 | ||
| 264 | 1 |
_aBerlin, Heidelberg _bSpringer International Publising _c2010 |
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| 300 |
_a1 recurso en línea (XIII, 788 páginas) _b |
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| 336 |
_atexto _btxt _2rdacontent |
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| 337 |
_aelectrónico _bc _2rdamedia |
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| 338 |
_arecurso electrónico _bcr _2rdacarrier |
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| 347 |
_aarchivo de texto _bPDF |
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| 490 | 0 |
_aSpringer Protocols Handbooks _x1949-2456 |
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| 505 | 0 | _aCloning of the Antigen-binding Site from Hybridoma -- Cloning of Variable Domains from Mouse Hybridoma by PCR -- Coning Hybridoma cDNA by RACE -- Construction of scFv Fragments from Hybridoma or Spleen Cells by PCR Assembly -- Generation of Antibody Repertoires -- Mouse Immune Libraries for the Generation of ScFv Fragments Directed Against Human Cell Surface Antigens -- Human Antibody Gene Libraries -- Synthetic Antibody Libraries -- Immune Libraries from Nonhuman Primates (NHP) -- Generation of Rabbit Immune Libraries -- Selection of Antibody Fragments from Combinatorial Libraries -- Immunotube Selections -- Phage Display and Selection in Microtitre Plates -- Phage Display and Selections on Biotinylated Antigens -- Phage Display and Subtractive Selection on Cells -- Selection of Phage Antibody Libraries for Binding and Internalization into Mammalian Cells -- Improving Phage Display Throughput by Using Hyperphage, Miniaturized Titration and pVIII (g8p) ELISA -- Yeast Display and Selections -- The Generation of Transgenic Mice Expressing Human Antibody Repertoires -- Selection of Antibody Fragments by Means of the Filter-Sandwich Colony Screening Assay -- Semi-automated Magnetic Bead-Based Antibody Selection from Phage Display Libraries -- Engineering and Production of Immunoglobulins -- Aspects of Isotype Selection -- Generation of Heavy and Light Chains (Chimeric Antibodies) -- Humanising Antibodies by CDR Grafting -- Humanization by Resurfacing -- Human Antibodies by Guided Selection -- In Silico De-Immunization -- Affinity Maturation by Chain Shuffling and Site Directed Mutagenesis -- Affinity Maturation by Random Mutagenesis and Phage Display -- Engineering of the Fc Region for Improved PK (FcRn Interaction) -- Antibody-Dependent Enzyme Prodrug Therapy (ADEPT) -- Production of Recombinant Human IgG Antibodies in the Baculovirus Expression System -- Expression of IgA Molecules in Mammalian Cells -- Antibody Characterization -- Expression of Complete Antibodies in Transgenic Plants -- Expression of Full Length Monoclonal Antibodies (mAb) in Algal Chloroplast -- Expression of IgG Antibodies in Mammalian Cells -- Protein A/G Chromatography -- Epitope Analysis Using Synthetic Peptide Repertoires Prepared by SPOT Synthesis Technology -- Epitope Mapping by Printed Peptide Libraries -- Antibody Epitope Mapping Using Yeast Display -- Size Exclusion Chromatography -- Structural Characterization of Antibodies by Mass Spectrometry -- Antibody Glycans Characterization -- Affinity Measurements by Competition ELISA -- Anti-Histidine Antibodies as Tools for Reversible Capturing of His-Tagged Fusion Proteins for Subsequent Binding Analysis -- Affinity Measurements Using Quartz Crystal Microbalance (QCM) -- Affinity Measurements with Radiolabeled Antibodies -- Neutralization Tests -- Functional Characterization of Antibodies Neutralizing Soluble Factors In Vitro and In Vivo -- Competitive ELISA -- Quantification of Human IgG and Related Fc Fusion Proteins by a Human IgG/Fc Capture ELISA -- Determination of Fc-Mediated Antibody-Effector Functions by Chromium Release Assay -- Binding Studies with Flow Cytometry. | |
| 520 | _aAntibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. Antibody Engineering provides a comprehensive toolbox covering the well-established basics but also many exciting new techniques. The protocols reflect the latest "hands on" knowledge of key laboratories in this still fast-moving field. Newcomers will benefit from the proven step-by-step protocols, which include helpful practical advice; experienced antibody engineers will appreciate the new ideas and approaches. The book is an invaluable resource for all those engaged in antibody research and development. | ||
| 700 | 1 |
_aKontermann, Roland E _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt |
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| 700 | 1 |
_aDübel, Stefan _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt |
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| 776 | 0 | 8 |
_iPrinted edition: _z9783642011450 |
| 776 | 0 | 8 |
_iPrinted edition: _z9783642011436 |
| 776 | 0 | 8 |
_iPrinted edition: _z9783662495940 |
| 856 | 4 | 0 |
_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-3-642-01144-3 _z(usuarios Universidad Europea de Valencia) |
| 942 |
_2lcc _cLE |
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| 988 | _aSpringer_Protocols_2010 | ||
| 999 |
_c234954 _d234954 |
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