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020 _a9781493969722
024 7 _a10.1007/978-1-4939-6972-2
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aHeterologous Protein Production in CHO Cells
_bMethods and Protocols
_cedited by Paula Meleady.
250 _a1st edition 2017
264 1 _aNew York, NY
_bSpringer International Publishing
_c2017
300 _a1 recurso en línea (X, 252 páginas)
_b35 ilustraciones, 28 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1603
505 0 _aStrategies and Considerations for Improving Expression of 'Difficult to Express' Proteins in CHO Cells -- Glycoengineering of CHO Cells to Improve Product Quality -- Large-Scale Transient Transfection of Chinese Hamster Ovary Cells in Suspension -- Cloning of Single-Chain Antibody Variants by Overlap-Extension PCR for Evaluation of Antibody Expression in Transient Gene Expression -- Anti-Apoptosis Engineering for Improved Protein Production from CHO Cells -- Conditional Knockdown of Endogenous MicroRNAs in CHO Cells using TET-ON-SanDI Sponge Vectors -- Application of CRISPR/Cas9 Genome Editing to Improve Recombinant Protein Production in CHO Cells -- Improved CHO Cell Line Stability and Recombinant Protein Expression During Long-Term Culture -- Selection of High-Producing Clones using FACS for CHO Cell Line Development -- The 'Omics Revolution in CHO Biology: Roadmap to Improved CHO Productivity -- A Bioinformatics Pipeline for the Identification of CHO Cell Differential Gene Expression from RNA-Seq Data -- Filter-Aided Sample Preparation (FASP) for Improved Proteome Analysis of Recombinant Chinese Hamster Ovary Cells -- Phosphopeptide Enrichment and LC-MS/MS Analysis to Study the Phosphoproteome of Recombinant Chinese Hamster Ovary Cells -- Engineer Medium and Feed for Modulating N-Glycosylation of Recombinant Protein Production in CHO Cell Culture -- Glycosylation Analysis of Therapeutic Glycoproteins Produced in CHO Cells -- Characterization of Host Cell Proteins (HCPs) in CHO Cell Bioprocesses.
520 _aThis volume discusses protocols that cover genetic manipulation of Chinese hamster ovary (CHO) cells for recombinant protein production, and protocols for the characterization of CHO cells using 'omic approaches. This book also explores methods that discuss the genome editing tool, CRISPR/Cas9, and the characterization of recombinant protein products, such as glycosylation and host cell protein analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Heterologous Protein Production in CHO Cells: Methods and Protocols is a valuable resource for scientists and researchers who are interested in further studying cell production in CHO cells.
700 1 _aMeleady, Paula
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493969715
776 0 8 _iPrinted edition:
_z9781493969739
776 0 8 _iPrinted edition:
_z9781493983568
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6972-2
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2017
999 _c234957
_d234957