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001			234960
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008			140722s2014 xxu| s |||| 0|eng d
020			_a9781493911394
024	7		_a10.1007/978-1-4939-1139-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aNotch Signaling _bMethods and Protocols _cedited by Hugo J. Bellen, Shinya Yamamoto.
250			_a1st edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (XI, 351 páginas) _b59 ilustraciones, 46 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1187
505	0		_aIntroduction to Notch Signaling -- Genetic Screens to Identify New Notch Pathway Mutants in Drosophila -- Structure-function Analysis of Drosophila Notch using Genomic Rescue Transgenes -- Overview of Genetic Tools and Techniques to Study Notch Signaling in Mice -- Immunohistochemical Tools and Techniques to Visualize Notch in Drosophila melanogaster -- Antibody Uptake Assay and in vivo Imaging to Study Intracellular Trafficking of Notch and Delta in Drosophila -- Tracking Trafficking of Notch and its Ligands in Mammalian Cells -- Visualizing Notch Signaling in vivo in Drosophila Tissues -- Monitoring Notch Activity in the Mouse -- Notch Signaling Assays in Drosophila Cultured Cell Lines -- Monitoring Notch Activation in Cultured Mammalian Cells: Transcriptional Reporter Assays -- Monitoring Notch Activation in Cultured Mammalian Cells: Luciferase Complementation Imaging Assays -- Visualization of Notch Signaling Oscillation in Cells and Tissues -- Proteomic Analysis of the Notch Interactome -- Bacterial Expression and in vitro Refolding of Limited Fragments of the Notch Receptor and its Ligands -- Analyzing the Post-translational Modification Status of Notch using Mass Spectrometry -- Assay to Probe Proteolytic Processing of Notch by γ-secretase -- Analyzing the Nuclear Complexes of Notch Signaling by Electrophoretic Mobility Shift Assay -- Identifying Direct Notch Transcriptional Targets using the GSI-washout Assay -- Probing the Epigenetic Status at Notch Target Genes -- Notch-ligand Binding Assays in Drosophila Cells -- Modeling Notch Signaling: A Practical Tutorial -- Small Molecules that Inhibit Notch Signaling -- Application and Evaluation of Anti-Notch Antibodies to Modulate Notch Signaling -- Application of Anti-ligand Antibodies to Inhibit Notch Signaling.
520			_aNotch Signaling: Methods and Protocols focuses on molecular, biochemical, and bioinformatics aspects of Notch signaling. Chapters analyze the Notch interactome, post-translational modifications of Notch, ligand binding assays, methods to assess proteolytic cleavage, and transcriptional targets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Notch Signaling: Methods and Protocols is a valuable contribution to hundreds of labs and thousands of scientists who pursue this research area with vigor.
700	1		_aBellen, Hugo J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aYamamoto, Shinya _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493911400
776	0	8	_iPrinted edition: _z9781493911387
776	0	8	_iPrinted edition: _z9781493955107
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1139-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2014
999			_c234960 _d234960