000			03372nam a22003615i 4500
001			234970
003			ES-VaUE
005			20221220020635.0
007			cr nn 008mamaa
008			150713s2015 xxu| s |||| 0|eng d
020			_a9781493928743
024	7		_a10.1007/978-1-4939-2874-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aCarbohydrate-Based Vaccines _bMethods and Protocols _cedited by Bernd Lepenies.
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XI, 255 páginas) _b73 ilustraciones, 21 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1331
505	0		_aCarbohydrate-based Vaccines: An Overview -- Glycans as Vaccine Antigens and Adjuvants: Immunological Considerations -- The Glycan Array Platform as a Tool to Identify Carbohydrate Antigens -- Antibody-carbohydrate Recognition from Docked Ensembles using the AutoMap Procedure -- Generation of Monoclonal Antibodies against Defined Oligosaccharide Antigens -- Murine Whole-Blood Opsonophagocytosis Assay to Evaluate Protection by Antibodies Raised Against Encapsulated Extracellular Bacteria -- Determination of N-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry and Ion Mobility -- N-Glycosylation Fingerprinting of Viral Glycoproteins by xCGE-LIF -- Temporary Conversion of Protein Amino Groups To Azides: A Synthetic Strategy For Glycoconjugate Vaccines -- Gold Nanoparticles as Carriers For Synthetic Glycoconjugate Vaccines -- Identification and Characterization of Carbohydrate-Based Adjuvants -- Characterization of Carbohydrate Vaccines by NMR Spectroscopy Francesco Berti and Neil Ravenscroft -- Characterisation of Capsular Polysaccharides and Their Glycoconjugates by Hydrodynamic Methods -- Glycoconjugate Vaccines: The Regulatory Framework.
520			_a This volume summarizes current cutting-edge methods related to carbohydrate-based vaccines, from the identification of a suitable carbohydrate antigen via the preparation of glycoconjugate vaccines to the characterization of vaccine candidates for their use in pre-clinical and clinical studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Carbohydrate-Based Vaccines: Methods and Protocols aims to be a valuable manual that will facilitate research in the field of carbohydrate-based vaccines.
700	1		_aLepenies, Bernd _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493928736
776	0	8	_iPrinted edition: _z9781493928750
776	0	8	_iPrinted edition: _z9781493949199
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2874-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c234970 _d234970