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001			234972
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008			100301s2005 xxu| s |||| 0|eng d
020			_a9781592598748
024	7		_a10.1385/1592598749 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aAmyloid Proteins _cedited by Einar M. Sigurdsson.
250			_a1st edition 2005
264		1	_aTotowa, NJ _bHumana Press _c2005
300			_a1 recurso en línea (408 páginas) _b156 ilustraciones, 1 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v299
505	0		_aIn Vitro Assays -- Preparation of Aggregate-Free,Low Molecular Weight Amyloid? for Assembly and Toxicity Assays -- Determination of Peptide Oligomerization State Using Rapid Photochemical Crosslinking -- In Vitro Preparation of Prefibrillar Intermediates of Amyloid-? and ? Synuclein -- Purification of Recombinant Tau Protein and Preparation of Alzheimer-Paired Helical Filaments In Vitro -- Cyclic Amplification of Protein Misfolding and Aggregation -- X-Ray Diffraction Studies of Amyloid Structure -- Molecular Electron Microscopy Approaches to Elucidating the Mechanisms of Protein Fibrillogenesis -- Time -Lapse Atomic Force Microscopy in the Characterization of Amyloid-Like Fibril Assembly and Oligomeric Intermediates -- Fourier Transform Infrared and Circular Dichroism Spectroscopies for Amyloid Studies -- Quasielastic Light Scattering for Protein Assembly Studies -- Intrinsic Fluorescent Detection of Tau Conformation and Aggregation -- Quantitative Measurement of Fibrillogenesis by Mass Spectrometry -- Cell Culture Assays -- Isolation and Culturing of Human Vascular Smooth Muscle Cells -- Murine Cerebrovascular Cells as a Cell Culture Model for Cerebral Amyloid Angiopathy -- Purification of Human Wild-Type or Variant Cystatin C From Conditioned Media of Transfected Cells -- Prion Propagation in Cell Culture -- In Vivo-Related Assays -- Preparation and Propagation of Amyloid-Enhancing Factor -- Purification of Amyloid Protein AA Subspecies From Amyloid-Rich Human Tissues -- Purification of Transthyretin and Transthyretin Fragments From Amyloid-Rich Human Tissues -- Extraction and Chemical Characterization of Tissue-Deposited Proteins From Minute Diagnostic Biopsy Specimens -- Tissue Processing Prior to Protein Analysis and Amyloid-ß Quantitation -- ELISA Method for Measurement of Amyloid-ß Levels -- Histological Staining of Amyloid-ß in Mouse Brains -- The Mouse Model for Scrapie -- Radiolabeling of Amyloid-ß Peptides -- In Vivo Imaging of Amyloid-ß Deposits in Mouse Brain With Multiphoton Microscopy -- Magnetic Resonance Imaging of Amyloid Plaques in Transgenic Mice.
520			_aIn recent years, various in vitro and in vivo techniques, transgenic technologies, and novel animal models have been developed to understand amyloid diseases-such as Alzheimer's disease and some forms of diabetes-and to screen for drugs that can block amyloid formation and/or disassemble its associated fibrils. In Amyloid Proteins: Methods and Protocols, internationally known researchers describe in step-by-step detail their best techniques for studying these types of proteins and their involvement in the etiology, pathogenesis, diagnosis, and therapy of amyloid diseases. The contributors provide proven methods for the preparation of amyloid and its precursors (oligomers and protofibrils), in vitro assays and analytical techniques for their study, and cell culture models and assays for the production of amyloid proteins. Additional chapters present readily reproducible techniques for amyloid extraction from tissue, its detection in vitro and in vivo, as well as nontransgenic methods for developing amyloid mouse models. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and highly practical, Amyloid Proteins: Methods and Protocols offers both skilled and novice investigators a proven collection of standard and novel techniques for studying amyloid proteins and peptides, as well as their relation to amyloid diseases.
700	1		_aSigurdsson, Einar M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617375026
776	0	8	_iPrinted edition: _z9781588293374
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592598749 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2005
999			_c234972 _d234972