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020 _a9781617795367
024 7 _a10.1007/978-1-61779-536-7
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aNeurotrophic Factors
_bMethods and Protocols
_cedited by Stephen D Skaper.
250 _a1st edition 2012
264 1 _aTotowa, NJ
_bHumana Press
_c2012
300 _a1 recurso en línea (XIV, 421 páginas)
_b68 ilustraciones, 10 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v846
505 0 _aThe Neurotrophin Family of Neurotrophic Factors - An Overview -- Neuronal Growth-Promoting and Inhibiting Cues in Neuroprotection and Neurodegeneration -- Culture of Rat Cerebellar Granule Neurons and Application to Identify Neuroprotective Agents -- Isolation and Culture of Neural Progenitor Cells from Rat Postnatal Cerebellum -- Culture of Rodent Cortical and Hippocampal Neurons -- Amyloid β-Peptide Neurotoxicity Assay Using Cultured Rat Cortical Neurons -- Culture of Neonatal Rodent Microglia, Astrocytes, and Oligodendrocytes from Cortex and Spinal Cord -- Central Nervous System Neuron-Glia Co-Culture Models -- Culture and Characterization of Rat Mesencephalic Dopaminergic Neurons -- Preparation of Adult Spinal Cord Motor Neuron Cultures Under Serum-Free Conditions -- Rodent Retinal Ganglion Cell Cultures -- Culture of Purified Glial Cell Populations from Optic Nerve -- Isolation and Culture of Rat Cone Photoreceptor Cells -- Rat Retina Pigmented Epithelial Cells -- Mammalian Growth Cone Turning Assays Identify Distinct Cell Signalling Mechanisms that Underlie Axon Growth, Guidance and Regeneration -- Culture of Dissociated Sensory Neurons from Dorsal Root Ganglia of Postnatal and Adult Rats -- Culture and Proliferation of Highly Purified Adult Schwann Cells from Rat, Dog, and Man -- Use of PC12 Cells and Rat Superior Cervical Ganglion Sympathetic Neurons as Models for Neuroprotective Assays Relevant to Parkinson's Disease -- Compartmented Chambers for Studying Neurotrophic Factor Action -- Preparation and Culture of Adrenal Chromaffin Cells -- Indirect Immunofluorescence Staining of Cultured Neural Cells -- Neurite Outgrowth Assessment Using High Content Analysis Methodology -- Dissociated Cell Culture for Testing Effects of Carbon Nanotubes on Neuronal Growth -- High-Resolution Imaging and Evaluation of Spines in Organotypic Hippocampal Slice Cultures -- Imaging Amyloid Precursor Protein In Vivo - An Axonal Transport Assay -- The Use of Specific AAV Serotypes to Stably Transduce  Primary CNS Neuron Cultures -- Preparation and Characterization of Biocompatible Chitosan Nanoparticles for Targeted Brain Delivery of Peptides.-  [3H]Serotonin Release Assay Using Antigen-Stimulated Rat Peritoneal Mast Cells -- Rat Hippocampal Slice Culture Models for the Evaluation of Neuroprotective Agents -- A 6-Hydroxydopamine In Vivo Model of Parkinson's Disease -- Brain Microdialysis in Freely Moving Animals -- Evaluating Motor Neuron Death in Neonatal Rats Subjected to Sciatic Nerve Lesion -- Rodent Spinal Cord Injury Model and Application of Neurotrophic Factors for Neuroprotection.
520 _aNervous system development evolves from the well-orchestrated processes of neural induction, cell proliferation, differentiation, cell migration, survival, and synapse formation. Among these environmental cues, neurotrophic factors are secreted proteins that promote neurite outgrowth, neuronal cell differentiation and survival both in vivo and in vitro. Nerve growth factor (NGF) is the founding and best characterized member of the neurotrophin family of neurotrophic polypeptides. Neurotrophic Factors: Methods and Protocols presents a selection of protocols and procedures which make use of cellular, tissue, and whole animal models which can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. Chapters cover a wide-range of topics such as dealing with the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia co-culture models, and cell-based assays for the evaluation of neuroprotective molecules, as well as assays which can be applied to the study of agents with neuroregenerative potential. Protocols describing viral- and nanoparticle-based delivery methods to neural cells are also presented, following by chapters dealing with organotypic slice culture protocols. Lastly, several chapters are dedicated to in vivo lesion models of relevance to nervous system pathology, which can be applied to the investigation of neurotrophic factors and peptides. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Neurotrophic Factors: Methods and Protocols seeks to serve both professionals and novices alike with its well-honed methodologies in an effort to further our knowledge of what has been described as the last frontier of science.
700 1 _aSkaper, Stephen D
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617795350
776 0 8 _iPrinted edition:
_z9781617795374
776 0 8 _iPrinted edition:
_z9781493962105
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-536-7
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2012
999 _c234973
_d234973