000			03478nam a22003495i 4500
001			234981
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007			cr nn 008mamaa
008			100301s1999 xxu| s |||| 0|eng d
020			_a9781592592012
024	7		_a10.1385/1592592015 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aElectron Microscopy Methods and Protocols _cedited by M. A. Nasser Hajibagheri.
250			_a1st edition 1999
264		1	_aTotowa, NJ _bHumana Press _c1999
300			_a1 recurso en línea (XI, 283 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v117
505	0		_aGeneral Preparation of Material and Staining of Sections -- Negative Staining of Thinly Spread Biological Particulates -- Preparation of Thin-Film Frozen-Hydrated/ Vitrified Biological Specimens for Cryoelectron Microscopy -- The Production of Cryosections Through Fixed and Cryoprotected Biological Material and Their Use in Immunocytochemistry -- High-Pressure Freezing for Preservation of High Resolution Fine Structure and Antigenicity for Immunolabeling -- The Application of LR Gold Resin for Immunogold Labeling -- Low-Temperature Embedding in Acrylic Resins -- Quantitative Aspects of Immunogold Labeling in Embedded and Nonembedded Sections -- Microwave Processing Techniques for Electron Microscopy: A Four-Hour Protocol -- Electron Microscopic Enzyme Cytochemistry -- In Situ Molecular Hybridization Techniques for Ultrathin Sections -- Preparation of the Fission Yeast Schizosaccharomyces pombe for Ultrastructural and Immunocytochemical Study -- Preparation of Double/Single-Stranded DNA and RNA Molecules for Electron Microscopy -- Applications of Electron Microscopy for Studying Protein-DNA Complexes -- X-Ray Microanalysis Techniques.
520			_aIn Electron Microscopy Methods and Protocols, well-practiced experts describe in detail the key electron microscopy techniques used for examining cells, tissue, biological macromolecules, molecular structure, and their interactions. With emphasis on cryotechniques for quantitative biological X-ray microanalysis, the book also includes those methods that use antibodies to locate proteins within cells and that prepare and analyze nucleic acids, proteins, and protein-nucleic acid complexes. Numerous immunogold labeling techniques for precise ultrastructural localization, distribution, and quantitation of macromolecules in cryo-fixed or chemically-fixed cells are described in sufficient detail to provide practical insight into their advantages and limitations. Electron Microscopy Methods and Protocols offers both newcomers and established researchers wanting to expand their repertoire of cutting-edge electron microscopy techniques-each optimized for reproducibility and robust results-today's gold-standard laboratory manual.
700	1		_aHajibagheri, M. A. Nasser _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9780896036406
776	0	8	_iPrinted edition: _z9781489943569
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592592015 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1999
999			_c234981 _d234981