000			04512nam a22003615i 4500
001			234985
003			ES-VaUE
005			20221220020636.0
007			cr nn 008mamaa
008			161206s2017 xxu| s |||| 0|eng d
020			_a9781493964246
024	7		_a10.1007/978-1-4939-6424-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aERK Signaling _bMethods and Protocols _cedited by Gerardo Jimenez.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XII, 440 páginas) _b40 ilustraciones, 21 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1487
505	0		_aHow Genetics has Helped Piece Together the MAPK Signaling Pathway -- In Vitro Enzyme Kinetics Analysis of EGFR -- High-Throughput Analysis of Mammalian Receptor Tyrosine Kinase Activation in Yeast Cells -- Structural Studies of ERK2 Protein Complexes -- Isolation and Characterization of Intrinsically Active (MEK-Independent) Mutants of Mpk1/Erk -- Assaying Activation and Subcellular Localization of ERK in Cells and Tissues -- Detection and Functional Analysis of SUMO-Modified MEK -- Single-Step Affinity Purification of ERK Signaling Complexes Using the Streptavidin-Binding Peptide (SBP) Tag -- High-Throughput In Vitro Identification of Direct MAPK/Erk Substrates -- Global Identification of ERK Substrates by Phosphoproteomics Based on IMAC and 2D-DIGE -- Analysis of Ras/ERK Compartmentalization by Subcellular Fractionation -- Cell-Based Assays to Study ERK Pathway/Caveolin1 Interactions -- The Nuclear Translocation of ERK -- Visualization of RAS/MAPK Signaling In Situ by the Proximity Ligation Assay (PLA) -- Measuring ERK Activity Dynamics in Single Living Cells Using FRET Biosensors -- Quantifying Tensile Force and ERK Phosphorylation on Actin Stress Fibers -- Co-Culture Activation of MAP Kinase in Drosophila S2 Cells -- Isolation of Mouse Embryonic Stem Cell Lines in the Study of ERK1/2 MAP Kinase Signaling -- 3D Organotypic Culture Model to Study Components of ERK Signaling -- Genetic Validation of Cell Proliferation via Ras-Independent Activation of the Raf/Mek/Erk Pathway -- Genome-Wide Analysis of RAS/ERK Signaling Targets -- Probing Chromatin Modifications in Response to ERK Signaling -- Analyzing pERK Activation during Planarian Regeneration -- Discovering Functional ERK Substrates Regulating Caenorhabditis elegans Germline Development -- Reconstructing ERK Signaling in the Drosophila Embryo from Fixed Images -- Using CRISPR-Cas9 to Study ERK Signaling in Drosophila -- Analyzing ERK Signal Dynamics during Zebrafish Somitogenesis -- Modeling RASopathies with Genetically Modified Mouse Models -- Dissecting Cell-Fate Determination through Integrated Mathematical Modeling of the ERK/MAPK Signaling Pathway.
520			_aThis volume provides a collection of techniques and approaches for the study of ERK signaling. It begins with a historical perspective of genetic and molecular discoveries, followed by chapters covering specific topics in a broad range of experimental systems, including in vitro assays of EGFR and ERK activities; proteomic and genome-wide analyses of ERK signaling targets; cell biological, genetic, quantitative and imaging approaches in cells and model organisms; and integrated mathematical modeling of the ERK pathway. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, ERK Signaling: Methods and Protocols will aid and stimulate further advances in the vibrant field of ERK signaling. < .
700	1		_aJimenez, Gerardo _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493964222
776	0	8	_iPrinted edition: _z9781493964239
776	0	8	_iPrinted edition: _z9781493981953
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6424-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c234985 _d234985