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008			170714s2017 xxu| s |||| 0|eng d
020			_a9781493971831
024	7		_a10.1007/978-1-4939-7183-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMicrobial Steroids _bMethods and Protocols _cedited by José-Luis Barredo, Ignacio Herráiz.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 373 páginas) _b47 ilustraciones, 27 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1645
505	0		_aSteroid Bioconversions -- Chemical Pathways of Corticosteroids, Industrial Synthesis from Sapogenins -- Genome Sequencing of Steroid Producing Bacteria with Illumina Technology -- Genome Sequencing of Steroid Producing Bacteria using Ion Torrent Technology and a Reference Genome -- RNA-Seq-Based Comparative Transcriptomics: RNA Preparation and Bioinformatics -- Intra- and Extra-Cellular Proteome Analyses of Steroid-Producer Mycobacteria -- Genetic Techniques for Manipulation of the Phytosterol Biotransformation Strain Mycobacteriumneoaurum NRRL B-3805 -- Identification and Characterization of the genes and Enzymes Belonging to the Bile Acid Catabolic Pathway in Pseudomonas -- Selection of Biodegrading Phytosterol Strains -- Stigmasterol Removal by an Aerobic Treatment System -- Production and Biotransformation of Phytosterol Microdispersions to Produce 4-Androstene-3, 17-Dione -- Β-Sitosterol Bioconversion to Androstenedione in Microtiter Plates -- Bioconversion of Phytosterols into Androstenedione by Mycobacterium -- Scale-Up of Phytosterols Bioconversion into Androstenedione -- Bioconversion of Phytosterols into Androstadienedione by Mycobacterium smegmatis CECT 8331 -- Process for Biotransformation of Androsta-4-ene-3, 17-Dione (4-AD) to Androsta-1, 4-Diene-3, 17-Dione (ADD) -- Laboratory-Scale Hydroxylation of Steroids by P450BM3 Variants -- Obtaining of 11α-Hydroxy and Rost-4-ene-3, 17-Dione from Natural Sterols -- Steroid 11-Alfa-Hydroxylation by the Fungi Aspergillus nidulans and Aspergillus ochraceus -- Biotransformation of DHEA into 7α, 15α-diOH-DHEA -- Stabilization of Enzymes by Using Thermophiles -- Biocatalysis of Steroids with Mycobacterium Sp. in Aqueous and Organic Media -- Downstream Process Synthesis for Microbial Steroids -- Analysis of Intermediates of Steroid Transformations in Resting Cells by Thin Layer Chromatography (TLC) -- A Ligand-Binding Assay to Measure the Affinity and Specificity of Sterol-Binding Proteins In Vitro. .
520			_aThis volume provides practical experimental laboratory protocols for a wide range of steroid bioconversions. The chapters in this book cover topics such as bioconversions and chemical synthesis pathways; strain characterization; bioconversion from sterols to androstenedione and androstadienedione; steroid hydroxylations; biocatalysis; and downstream processes to purify steroid intermediates. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Microbial Steroids: Methods and Protocols is a valuable resource for laboratory and industrial professionals. It is also useful for graduate students studying biotechnology, microbiology, genetics, and molecular biology.
700	1		_aBarredo, José-Luis _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aHerráiz, Ignacio _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493971824
776	0	8	_iPrinted edition: _z9781493971848
776	0	8	_iPrinted edition: _z9781493984107
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7183-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c234989 _d234989