000			04429nam a22003735i 4500
001			234993
003			ES-VaUE
005			20221220020637.0
007			cr nn 008mamaa
008			130427s2013 xxu| s |||| 0|eng d
020			_a9781627034265
024	7		_a10.1007/978-1-62703-426-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aChemokines _bMethods and Protocols _cedited by Astrid E. Cardona, Eroboghene E. Ubogu.
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (XI, 238 páginas) _b37 ilustraciones, 19 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1013
505	0		_aThe Fine Balance of Chemokines During Disease: Trafficking, Inflammation, and Homeostasis -- Chemokine-Dependent Signaling Pathways in the Peripheral Nervous System -- Multispectral Imaging and Automated Laser Capture Microdissection of Human Cortical Neurons: A Quantitative Study of CXCR4 Expression -- Chemokine Receptors and Neural Stem Cells -- Chemokine Receptor Interactions with Virus-Like Particles -- Chemokine Receptor Antagonist Development -- A Novel Approach to Quantify G Protein-Coupled Receptor Dimerization Equilibrium Using Bioluminescence Resonance Energy Transfer -- Unraveling Chemokine and Chemokine Receptor Expression Patterns Using Genetically Engineered Mice -- Zebrafish as a Model to Study Chemokine Function -- Inflammatory Macrophage Migration in Experimental Autoimmune Encephalomyelitis -- Visualizing Chemokine-Dependent T Cell Activation and Migration in Response to Central Nervous System Infection -- Epigenetic Regulation of Chemokine/Chemokine Receptor Expression -- Using Fluorescent Chemokine Uptake to Detect Chemokine Receptors by Fluorescent Activated Cell Sorting -- Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse Ear Preparations -- Studying Extracellular Signaling Utilizing a Glycoproteomic Approach: Lectin Blot Surveys, a First and Important Step.
520			_aChemokines constitute a large family of structurally similar cytokines that contain a signature of conserved cysteine residues joined by disulfide bridges.  Binding of chemokines to specific G protein-coupled receptors followed by downstream signaling defines their biological function.  Initially, chemoattraction was the key function linked to chemokines/chemokine receptors; however, in recent years, it has become clear that chemokine ligand-receptor interactions can also modulate cellular activation, survival, and proliferation, among other functions in homeostatic and diseased states.  Importantly, major advances in our understanding of chemokine biology have led to chemokine receptors becoming specific therapeutic targets with great potential.  In Chemokines: Methods and Protocols, expert researchers provide practical information regarding experimental models and state of the art protocols used to delineate chemokine/chemokine receptor function and their applications in health and disease.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Practical and easy to use, Chemokines: Methods and Protocols aims to reveal key protocols of functional and descriptive chemokine ligand/receptor assays that will be of practical significance to graduate students, post-doctoral fellows, trainees, and researchers in academia and industry.
700	1		_aCardona, Astrid E _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aUbogu, Eroboghene E _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627034272
776	0	8	_iPrinted edition: _z9781627034258
776	0	8	_iPrinted edition: _z9781493960057
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-426-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2013
999			_c234993 _d234993