000			04645nam a22003735i 4500
001			234994
003			ES-VaUE
005			20221220020637.0
007			cr nn 008mamaa
008			140317s2014 xxu| s |||| 0|eng d
020			_a9781627037884
024	7		_a10.1007/978-1-62703-788-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHuman Cytomegaloviruses _bMethods and Protocols _cedited by Andrew D. Yurochko, William E. Miller.
250			_a1st edition 2014
264		1	_aTotowa, NJ _bHumana Press _c2014
300			_a1 recurso en línea (XI, 368 páginas) _b40 ilustraciones, 22 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1119
505	0		_aHistory of the Molecular Biology of Cytomegalovirus -- Overview of Human Cytomegalovirus Pathogenesis -- Distinct Properties of Human Cytomegalovirus Strains and the Appropriate Choice of Strains for Particular Studies -- Use of Diploid Human Fibroblasts as a Model System to Culture/Grow, Study and Understand Human Cytomegalovirus Infection -- Use of Recombinant Approaches to Construct Human Cytomegalovirus Mutants -- The Use of Primary Human Cells (Fibroblasts, Monocytes and Others) to Assess Human Cytomegalovirus Function -- Human Hematopoietic Long-Term Culture (hLTC) for Human Cytomegalovirus Latency and Reactivation -- Analysis of Cytomegalovirus Binding/Entry-Mediated Events -- Use of 5-Ethynyl-2'-Deoxyuridine Labelling and Flow Cytometry to Study Cell Cycle-Dependent Regulation of Human Cytomegalovirus Gene Expression -- Methods for Studying the Function of Cytomegalovirus GPCRs -- Methods for the Detection of Cytomegalovirus in Glioblastoma Cells and Tissues -- Methods to Study the Nucleocytoplasmic Transport of Macromolecules with Respect to Their Impact on the Regulation of Human Cytomegalovirus Gene Expression -- Fluorescence-Based Laser Capture Microscopy Technology Facilitates Identification of Critical In Vivo Cytomegalovirus Transcriptional Programs -- Techniques for Characterizing Cytomegalovirus-Encoded miRNAs -- What We Have Learned From Animal Models of HCMV -- Rodent Models of Congenital Cytomegalovirus Infection -- Recent Approaches and Strategies in the Generation of Anti-Human Cytomegalovirus Vaccines -- Approaches for the Generation of New Anti-Cytomegalovirus Agents:  Identification of Protein-Protein Interaction Inhibitors and Compounds Against the HCMV IE2 Protein.
520			_aWith the significant medical role that human cytomegalovirus plays in human disease (from acute disease following primary infection, to chronic disease due to life-long viral persistence), the need to better understand human cytomegalovirus biology and pathogenesis is needed.  Human Cytomegaloviruses: Methods and Protocols is designed to be an inclusive document covering all of the techniques and approaches necessary to understand and study the pathobiology of human cytomegalovirus. Topics covered include the history of human cytomegaloviruses, techniques to culture and grow the virus in model cell types, the use of primary cells for the study of human cytomegalovirus pathogenesis, modern molecular techniques for assessing the biological consequences of viral infection, animal models for study of cytomegalovirus replication and numerous other topics of current interest. Written in the successful Methods in Molecular Biology series format, each chapter includes an introduction, list of necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Human Cytomegaloviruses: Methods and Protocols serves as a tool for basic scientists as well as clinical scientists with an interest in the basic fundamental aspects of viral gene expression and specific aspects of viral pathogenesis. .
700	1		_aYurochko, Andrew D _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aMiller, William E _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627037891
776	0	8	_iPrinted edition: _z9781627037877
776	0	8	_iPrinted edition: _z9781493962730
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-788-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2014
999			_c234994 _d234994