000			03953nam a22003735i 4500
001			234995
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007			cr nn 008mamaa
008			150105s2015 xxu| s |||| 0|eng d
020			_a9781493922727
024	7		_a10.1007/978-1-4939-2272-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aSite-Specific Protein Labeling _bMethods and Protocols _cedited by Arnaud Gautier, Marlon J. Hinner.
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XII, 267 páginas) _b72 ilustraciones, 34 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1266
505	0		_aHow FlAsH got its Sparkle:Historical Recollections of the Biarsenical-tetracysteine Tag -- Site-Specific Protein Labeling in the Pharmaceutical Industry - Experiences from Novartis Drug Discovery -- Getting Across the Cell Membrane: An Overview for Small Molecules, Peptides, and Proteins -- Considerations and Protocols for the Synthesis of Custom Protein Labeling Probes -- 2-Cyanobenzothiazole (CBT) Condensation for Site-Specific Labeling of Proteins at the Terminal Cysteine Residues -- Fluorescent Labeling for Patch Clamp Fluorometry (PCF) Measurements of Real-Time Protein Motion in Ion Channels -- Fluorescent Labeling Of SNAP-Tagged Proteins In Cells -- HaloTag Technology For Specific And Covalent Labeling Of Fusion Proteins -- Ligation Of Synthetic Peptides To Proteins Using Semisynthetic Protein Trans-Splicing -- Chemical-tag Labeling Of Proteins Using Fully Recombinant Split Inteins -- Phage Selection Assisted by Sfp Phosphopantetheinyl Transferase Catalyzed Site-Specific Protein Labeling -- Site-Specific Biotinylation Of Purified Proteins Using BirA.- Site-specific Labeling of Proteins via Sortase: Protocols for the Molecular Biologist -- BONCAT - Metabolic Labeling, Click Chemistry, and Affinity Purification Of Newly Synthesized Proteomes -- Genetic Encoding Of Unnatural Amino Acids For Labeling Proteins -- Labeling Proteins By Affinity-Guided DMAP Chemistry -- Ligand-Directed Tosyl Chemistry For Selective Native Protein Labeling In Vitro, In Cells, and In Vivo.
520			_aThis detailed volume provides in-depth protocols for protein labeling techniques and applications, with an additional focus on general background information regarding the design and generation of the organic molecules used for the labeling step. Chapters present a comprehensive overview on the most relevant and established labeling methodologies, and help researchers to choose the most appropriate labeling method for their biological question. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Site-Specific Protein Labeling: Methods and Protocols is designed to help scientists from all fields to get a better understanding of labeling techniques.
700	1		_aGautier, Arnaud _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aHinner, Marlon J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493922734
776	0	8	_iPrinted edition: _z9781493922710
776	0	8	_iPrinted edition: _z9781493944170
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2272-7 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c234995 _d234995